6 documents found, page 1 of 1
Impairment on nuclear maturation rate in oocytes from cows naturally infected b...
Mendes,Vívian R.A.; Costa,Eduardo P.; Queiroz,Vanessa L.D.; Silva Júnior,Abelardo; Alves,Saullo V.P.; Guimarães,José D.; Gomes,Lidiany L.ABSTRACT Bovine herpesvirus 1 (BoHV-1) is an important bovine pathogen that is responsible for causing respiratory diseases and reproductive failures. The presence of BoHV-1 in an in vitro embryo production system affects fertilization, maturation, and embryonic development. The objective of this study was to evaluate the developmental capacity of oocytes from naturally infected cows with no reproductive histor...
Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in Brazilian pati...
Carvalho,Isabel Azevedo; Schwarz,David Germano Gonçalves; Pietralonga,Pricila Aparecida Grasse; Faria,Ana Carolina Silva; Braga,Isis Freitas EspechitCONTEXT AND OBJECTIVE: Mycobacterium avium subsp. paratuberculosis (MAP) has attracted the interest of researchers because of similarities between paratuberculosis and Crohn's disease (CD). The aim of this study was to evaluate the frequency of MAP through cultures, histology and polymerase chain reaction (PCR) on intestinal biopsies from Brazilian CD patients. Quantitative real time PCR (qRT-PCR) was performed...
Cytokine gene expression and molecular detection of Mycobacterium avium subspec...
Schwarz,David G.G.; Pietralonga,Pricila A.G.; Souza,Marina C.C.; Carvalho,Isabel A.; Cruzeiro,Rosyane S.; Malaquias,Juaci V.; Benjamin,Laércio A.AbstractMycobacterium avium subspecies paratuberculosis (MAP) can infect ruminants and remain subclinical for long periods within herds. The identification of organs that are more susceptible to infection and the evaluation of cytokine expression at the site of infection are important to understand the pathogenesis of MAP. In this study, the probability of detection of MAP-DNA and the expression of cytokines in...
Retrospective study on Porcine circovirus-2 by nested pcr and real time pcr in ...
Silva,Fernanda Miquelitto Figueira da; Silva Júnior,Abelardo; Peternelli,Ethel Fernandes de Oliveira; Viana,Vinícius Winter; Chiarelli Neto,OrlandoPorcine circovirus-2 (PCV-2) infection is currently considered an important disease of swine. The pathogenic agent was first described in Brazil in 2000. This study detected the PCV-2 DNA in four Brazilian pig tissues collected between 1978 and 1979. This observation is the oldest description of this virus in Brazil.
Imunogenicidade de isolados de herpesvírus bovino 5 como candidatos à vacina
Souza,Luiz Felipe Lourenço de; Silva Júnior,Abelardo; Freitas,Carla Maria Batista de; Freitas,Ethel Cardoso de; Flores,Eduardo FurtadoO herpesvírus bovino 5 (BoHV-5) é o agente da menigoencefalite herpética bovina. A doença neurológica, associada à infecção pelo BoHV-5, apresenta altas taxas de letalidade em bovinos jovens e está disseminada no Brasil. A prevenção das perdas causadas pela infecção pelo herpesvírus está baseada, principalmente, na imunização dos animais. Nesse sentido, foi delineada uma comparação entre isolados de BoHV-5, bus...
Development and evaluation of a recombinant DNA vaccine candidate expressing po...
Silva Júnior,Abelardo; Castro,Luiza A.; Chiarelli Neto,Orlando; Silva,Fernanda M.F.; Vidigal,Pedro M.P.; Moraes,Mauro P.; Almeida,Márcia R.Porcine circovirus 2 (PCV2) is generally associated with the porcine circovirosis syndrome, which is considered an important disease of swine and has potentially serious economic impact on the swine industry worldwide. This article describes the construction of a recombinant plasmid expressing the PCV2 structural protein and the evaluation of cellular and humoral immune responses produced by this recombinant va...