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Assessing the virulence potential of different Listeria monocytogenes Clonal Co...

Sousa, Mariana; Silva, Sónia; Araújo, Daniela; Castro, Joana; Almeida, Gonçalo; Ventura, Catarina; Magalhães, Rui; Ferreira, Vânia; Teixeira, Paula

Introduction and Objective: Listeria monocytogenes is a pathogen causes infection mainly through food contamination. This species is well-known for its diversity, exhibiting differential virulence potential within strains from different clonal complexes (CCs). These can be defined as hypervirulent CCs, that are highly frequent among clinical cases and cause severe clinical outcomes (i.e. maternal-neonatal and c...


Assessing the virulence potential of different Listeria monocytogenes clonal co...

Sousa, Mariana; Silva, Sónia Carina; Araújo, Daniela Eira; Castro, Joana Isabel Reis; Almeida, Gonçalo; Ventura, Catarina; Magalhães, Rui

Listeria monocytogenes is a highly diverse species, exhibiting differential virulence potential within strains from different clonal complexes (CCs). Hypervirulent CCs strains tend to be associated with higher frequency in clinical cases and severe outcomes, while hypovirulent CCs are characterized by a reduced level of virulence and are often associated with food-related contamination. Recently, researchers ha...


External Quality Assessment in Laboratory Safety Area (PNAEQ-1S Segurança Labor...

Ventura, Catarina; Cardoso, Ana; Pires, Ana; Faria, Ana Paula

Introduction: The National External Quality Assessment Program (PNAEQ) provides tools for carrying out external quality assessment in Laboratory Safety area, since 2010. These tools have changed over the years, but all of them allow the identification of occurrences and/or critical points in the operation, installations or equipment, that could have impact on the safety of patients and workers, beyond prioritiz...


Results of the Portuguese EQA program for molecular detection of SARS-CoV-2, 20...

Correia, Helena; Silva, Susana; Ventura, Catarina; Melo, Aryse; Guiomar, Raquel; Faria, Ana Paula

The initial strategy for containing the spread of SARS CoV 2 was the prompt identification of COVID 19 cases. National Reference laboratory implemented the biomolecular assays to detect SARS-CoV-2. In June 2020 the Portuguese National External Quality Assessment Program (PNAEQ) in collaboration with National Reference Laboratory for Influenza and Other Respiratory Viruses (NRL) organized the first National Exte...


Vantagens de Participação no Programa Nacional de Avaliação Externa da Qualidad...

Ventura, Catarina; Cardoso, Ana; Correia, Helena; Cordeiro, Marina; Castro, Paulo; Pereira, Edna; Faria, Ana Paula

Introdução: O Programa Nacional de Avaliação Externa da Qualidade (PNAEQ) foi implementado no Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal, em 1978. A participação é voluntária, e permite monitorizar e implementar a melhoria da qualidade analítica, e de um modo independente, garantir a qualidade dos serviços prestados. Objetivo: Apresentação das vantagens que a participação nos programas de Avalia...


External Quality Assessment in Laboratory Safety Area (PNAEQ-1S Segurança Labor...

Ventura, Catarina; Cardoso, Ana; Pires, Ana; Faria, Ana

Introduction: The National External Quality Assessment Program (PNAEQ) provides tools for carrying out external quality assessment in Laboratory Safety area. These tools allow the identification of occurrences and/or critical points in the operation, installations or equipment, that could have impact on the safety of patients and workers, beyond prioritize the necessary improvement actions. Objective: Presentat...


Pre analytical Checklist: a relevant tool for External Quality Assessment (EQA)

Ventura, Catarina; Jesus, Lúcia de; Cardoso, Ana; Silva, Susana; Faria, Ana Paula

According to ISO 15189 2012 laboratory shall participate in an external quality assessment (programme and implement the corrective actions when necessary The EQA programs should have the effect of checking the entire analytical process, including pre and post examination procedures The National Program for External Quality Assessment (provides since 2007 EQA pre analytical schemes, including a checklist since 2...


Overview of PNAEQ clinical participants during COVID-19 pandemic

Faria, Ana Paula; Correia, Helena; Ventura, Catarina; Cardoso, Ana; Pereira, Edna; Aldeia, Claudia; Júlio, Claudia; Miranda, Armandina; Silva, Cândido

External Quality Assessment Schemes are a crucial tool to identify and evaluate laboratory performance. COVID 19 pandemic poses a challenge for laboratories, since they own a critical role in the diagnosis, prognosis and therapeutic monitoring The laboratory practice in stressful conditions may increase errors due to several factors. The main objective of this study was to evaluate and compare the performance o...


External Quality Assessment Pilot Study for the detection of Candida auris

Veríssimo, Cristina; Correia, Helena; Ventura, Catarina; Sabino, Raquel; Faria, Ana Paula

Candida auris, identified for the first time in 2009, is a multi-resistant emergent yeast that causes nosocomial outbreaks all over the world. This species can be misidentified with other Candida species, if the method used for yeasts’ identification (biochemical / mass spectrometry (MALDI-TOF-MS) has not been updated in order to contain the profile of this species. Aiming to raise the awareness about the risk ...


Results of Portuguese Extra-Analytical Evaluation on SARS-CoV-2 in 2020 schemes

Ventura, Catarina; Cardoso, Ana; Correia, Helena; Guiomar, Raquel; Melo, Aryse; Faria, Ana Paula

The Portuguese National External Quality Assessment Program (PNAEQ) assembles 3 schemes of extra-analytical issues since 2007 (Pre and Post-Analytical) and 2010 (Laboratory Safety). In 2020, due to the new Coronavirus (SARS-CoV-2), PNAEQ introduced a new SARS-CoV-2 molecular detection scheme with two rounds (May and November). In addition, were included questions in the 3 extra-analytical schemes concerning SAR...


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