8 documents found, page 1 of 1
Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons
Costa, Rui O.; Martins, Helena; Martins, Luís F.; Cwetsch, Andrzej W; Mele, Miranda; Pedro, Joana R.; Tomé, Diogo; Jeon, Noo Li; Cancedda, LauraRibosomes and a subset of cellular mRNAs are trafficked into axons of developing neurons. The axonal localization of translational machinery allows new proteins to be rapidly and locally synthesized during axonal growth and pathfinding. However, in mature neurons, axonal ribosomes are significantly reduced or even absent. The mechanism that elicits this removal is currently unknown. Here, we demonstrate that sy...
Alterations in GABAA-Receptor Trafficking and Synaptic Dysfunction in Brain Dis...
Mele, Miranda; Costa, Rui O.; Duarte, Carlos B.GABAA receptors (GABAAR) are the major players in fast inhibitory neurotransmission in the central nervous system (CNS). Regulation of GABAAR trafficking and the control of their surface expression play important roles in the modulation of the strength of synaptic inhibition. Different pieces of evidence show that alterations in the surface distribution of GABAAR and dysregulation of their turnover impair the a...
The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA M...
Leal, Graciano; Comprido, Diogo; Luca, Pasqualino De; Morais, Eduardo Manuel Firmo; Rodrigues, Luís; Mele, Miranda; Santos, Ana R.; Costa, Rui O.Brain-derived neurotrophic factor (BDNF) is an important mediator of long-term synaptic potentiation (LTP) in the hippocampus. The local effects of BDNF depend on the activation of translation activity, which requires the delivery of transcripts to the synapse. In this work, we found that neuronal activity regulates the dendritic localization of the RNA-binding protein heterogeneous nuclear ribonucleoprotein K ...
Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
Martins, Luís F.; Costa, Rui O.; Pedro, Joana R.; Aguiar, Paulo; Serra, Sofia C.; Teixeira, Fabio G.; Sousa, Nuno; Salgado, António J.Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance in central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of cells required for transplantation and low survival rate when transplanted into the central nervous system (CNS). In line with this, MSCs secretome which present on its c...
Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
Martins, Luís F.; Costa, Rui O.; Pedro, Joana R.; Aguiar, Paulo; Serra, Sofia Cravino; Teixeira, Fábio Gabriel Rodrigues; Sousa, Nuno; Salgado, A. J.Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance in central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of cells required for transplantation and low survival rate when transplanted into the central nervous system (CNS). In line with this, MSCs secretome which present on its c...
The RNA-binding protein hnRNP K mediates the effect of BDNF on dendritic mRNA m...
Leal, Graciano; Comprido, Diogo; Luca, Pasqualino de; Morais, Eduardo; Rodrigues, Luís; Mele, Miranda; Santos, Ana R.; Costa, Rui O.; Pinto, Maria JoanaBrain-derived neurotrophic factor (BDNF) is an important mediator of long-term synaptic potentiation (LTP) in the hippocampus. The local effects of BDNF depend on the activation of translation activity, which requires the delivery of transcripts to the synapse. In this work, we found that neuronal activity regulates the dendritic localization of the RNA-binding protein heterogeneous nuclear ribonucleoprotein K ...
Visualizing K48 Ubiquitination during Presynaptic Formation By Ubiquitination-I...
Pinto, Maria J.; Pedro, Joana R.; Costa, Rui O.; Almeida, Ramiro D.In recent years, signaling through ubiquitin has been shown to be of great importance for normal brain development. Indeed, fluctuations in ubiquitin levels and spontaneous mutations in (de)ubiquitination enzymes greatly perturb synapse formation and neuronal transmission. In the brain, expression of lysine (K) 48-linked ubiquitin chains is higher at a developmental stage coincident with synaptogenesis. Neverth...
Visualizing K48 Ubiquitination during Presynaptic Formation By Ubiquitination-I...
Pinto, Maria J.; Pedro, Joana R.; Costa, Rui O.; Almeida, Ramiro D.In recent years, signaling through ubiquitin has been shown to be of great importance for normal brain development. Indeed, fluctuations in ubiquitin levels and spontaneous mutations in (de)ubiquitination enzymes greatly perturb synapse formation and neuronal transmission. In the brain, expression of lysine (K) 48-linked ubiquitin chains is higher at a developmental stage coincident with synaptogenesis. Neverth...