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Genetic Diversity of Pseudomonas syringae pv. actinidiae: Seasonal and Spatial ...

Figueira, Daniela; Garcia, Eva; Ares, Aitana; Tiago, Igor Clemente; Veríssimo, António; Costa, Joana

Pseudomonassyringae pv. actinidiae (Psa) is a gram-negative bacterium responsible for the bacterial canker in Actinidia chinensis var. deliciosa and A. chinensis var. chinensis, a quarantine organism threatening the kiwifruit industry sustainability. The present study aimed to determine the genetic structure of the endophytic and epiphytic populations of Psa isolated from four different Portuguese orchards with...


In memoriam. Farewell Milton

Manaia, Célia M.; Albuquerque, Luciana; Veríssimo, António; Morais, Paula V.; Nunes, Olga C.


Differences in Virulence Between Legionella pneumophila Isolates From Human and...

Sousa, Patrícia S.; Silva, Inês N.; Moreira, Leonilde M; Veríssimo, António; Costa, Joana

Legionella pneumophila is a ubiquitous bacterium in freshwater environments and in many man-made water systems capable of inducing pneumonia in humans. Despite its ubiquitous character most studies on L. pneumophila virulence focused on clinical strains and isolates from man-made environments, so little is known about the nature and extent of virulence variation in strains isolated from natural environments. It...


The recombination dynamics of Staphylococcus aureus inferred from spA gene

Santos-Júnior, Célio D.; Veríssimo, António; Costa, Joana

Background: Given the role of spA as a pivotal virulence factor decisive for Staphylococcus aureus ability to escape from innate and adaptive immune responses, one can consider it as an object subject to adaptive evolution and that variations in spA may uncover pathogenicity variations. Results: The population genetic structure was deduced from the extracellular domains of SpA gene sequence (domains A-E and the...


Intragenic Recombination Has a Critical Role on the Evolution of Legionella pne...

Costa, Joana; Teixeira, Paulo Gonçalves; d’Avo, Ana Filipa; Junior, Célio Santos; Veríssimo, António

SidJ is a Dot/Icm effector involved in the trafficking or retention of ER-derived vesicles to Legionella pneumophila vacuoles whose mutation causes an observable growth defect, both in macrophage and amoeba hosts. Given the crucial role of this effector in L. pneumophila virulence we investigated the mechanisms shaping its molecular evolution. The alignment of SidJ sequences revealed several alleles with amino ...


Amplified ribosomal DNA restriction analysis as a routine tool to assess toxica...

Santos, Miguel J. G.; Sousa, José Paulo; Tiago, Igor; Veríssimo, António; Lemos, Marco F. L.

Changes in saprophytic organism's gut microbial communities may present a threat to organic matter breakdown which can ultimately lead to soil function impairment. In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This ...


Amplified ribosomal DNA restriction analysis as a routine tool to assess toxica...

Santos, Miguel J. G.; Sousa, José Paulo; Tiago, Igor; Veríssimo, António; Lemos, Marco

Changes in saprophytic organism's gut microbial communities may present a threat to organic matter breakdown which can ultimately lead to soil function impairment. In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This ...

Date: 2011   |   Origin: IC-online

Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt ev...

Albuquerque, Luciana; Tiago, Igor; Taborda, Marco; Nobre, M. Fernanda; Veríssimo, António; Costa, Milton S. da

A low-G+C, Gram-positive isolate, designated strain CVS-8(T), was isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago. This organism was found to be a catalase- and oxidase-positive, non-motile, spore-forming, aerobic, curved rod-shaped organism with an optimum growth temperature of about 35-37 degrees C and an optimum pH between 7.5 and 8.0. Optimal growth occurred in m...


Tepidimonas thermarum sp. nov., a new slightly thermophilic betaproteobacterium...

Albuquerque, Luciana; Tiago, Igor; Veríssimo, António; Costa, Milton S.

Several non-pigmented bacterial isolates, with an optimum growth temperature of about 50-55 °C, were recovered from the Elisenquelle at Aachen, Germany. Phylogenetic analysis of the 16S rRNA gene sequence of strains AA-1T and AA-2 indicated that these organisms represent a new species of the genus Tepidimonas. The major fatty acids of strains AA-1T and AA-2 are 16:0 and 16:1 [omega]7c. Ubiquinone 8 is the major...


Antibiotic resistance of enterococci and related bacteria in an urban wastewate...

Silva, Miguel Ferreira da; Tiago, Igor; Veríssimo, António; Boaventura, Rui A. R.; Nunes, Olga C.; Manaia, Célia M.

The main objective oflhis work was to study the ecology of enterooocci and related bacteria in raw and treated wastewater from a treatment plant receiving domestic and pretreated industrial effluents in order to assess the influence of treatment on the prevalence of antibiotic resistance phenotypes among this group ofbacteria. The predominant species found in the raw wastewater-were Erztercaccus hirae, Entercoc...


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