5 documents found, page 1 of 1
Whole-genome sequencing as a tool for studying the microevolution of drug-resis...
Diniz, Jaciara; von Groll, Andrea; Unis, Gisela; Dalla-Costa, Elis Regina; Rosa Rossetti, Maria Lúcia; Vianna, Júlia Silveira; Ramos, Daniela FernandesTreatment of drug-resistant tuberculosis requires extended use of more toxic and less effective drugs and may result in retreatment cases due to failure, abandonment or disease recurrence. It is therefore important to understand the evolutionary process of drug resistance in Mycobacterium tuberculosis. We here in describe the microevolution of drug resistance in serial isolates from six previously treated patie...
Helicobacter pylori infection and associated factors
Vianna, Júlia Silveira; Ramis, Ivy Bastos; Silva Junior, Lande Vieira da; Halicki, Priscila Cristina Bartolomeu; Gauterio, Thaisa BozzettiBackground and Objectives: Helicobacter pylori is linked to gastroduodenal pathologies. To determine the frequency and potential risk factors of the H. pylori infection. Material and methods: A cross-sectional study was conducted, including 227 patients, submitted to upper gastrointestinal endoscopy. A questionnaire was applied to the patients, before endoscopy. The biopsy specimens were obtained from the antru...
The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in ...
Vianna, Júlia Silveira; Ramis, Ivy Bastos; Ramos, Daniela Fernandes; Gastal, Otávio Leite; Silva, Renato Azevedo da; Gonçalves, Carla VitolaIn this study, we evaluated the mutations of Helicobacter pylori associated with resistance to clarithromycin and levofloxacin. Furthermore, based on the proposed interaction between antimicrobial resistance and pathogenicity, we correlated the mutation profiles of the strains with the presence of the pathogenicity gene cagA. We analyzed 80 gastric biopsy specimens from H. pylori-infected patients for point mut...
Helicobacter pylori pathogenicity genes, cytokine polymorphisms and environment...
Vianna, Júlia Silveira; Almeida da Silva, Pedro Eduardo; Bastos Ramis, IvyBackgrounds and objectives: Helicobacter pylori is a Gram-negative bacterium that colonizes the stomach of approximately 50% of the world´s human population. This microorganismis the major causal agent of gastritis and is an important risk factor for the development of peptic ulcer disease and gastric carcinoma. The factors that determine these diverse clinical outcomes are subject to continuous investigations ...
Genotypic analysis of Helicobacter pylori by Multiple-Locus Variable-Number Tan...
Halicki, Priscila Cristina Bartolomeu; Ramis, Ivy Bastos; Vianna, Júlia Silveira; Gautério, Thaísa Bozzetti; Groll, Andrea vonBackground and objectives: Despite its use as a genotyping method for different microorganisms, few studies have reported the use of Multiple-Locus Variable Number of Tandem Repeats Analysis (MLVA) for the analysis of the clonal diversity of Helicobacter pylori. The aim of this study was to determine the genetic variability of H. pylori strains by MLVA in southern Brazil. Methods: 95 H. pylori DNA samples were ...