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Ionic liquid-based nanoemulsion as a promising strategy to enhance the bioacces...

Sousa, Vítor; Lüdtke, Fernanda Luisa; Hijo, Ariel A. C. Toledo; Loureiro, Luís; Dias, Oscar; Vicente, A. A.; Pinheiro, A. C.; Geada, Pedro

Dunaliella salina is a unicellular halophile green microalga that presents the capacity to accumulate high amounts of β-carotene, a bioactive compound widely recognized by its health benefits. To fulfil its biological functions, however, β-carotene needs to be bioaccessible, which is challenging as its bioaccessibility is typically low. A promising strategy to overcome β-carotene's low bioaccessibility is its e...


Cellulose nanofibers as stabilizer of Pickering emulsions and reinforcement of ...

Chevalier, Raquel Costa; Vieira, Jorge Miguel Magalhães; Martins, Joana T.; Cunha, Rosiane Lopes; Vicente, A. A.

Cellulose nanofibers (CNF) can be used as biocompatible reinforcing agents in biodegradable films to be applied on food products, such as fruits, to protect them. Tomato is an extremely perishable fruit that can easily spoil due to inadequate handling or storage conditions in the post-harvest period, including contamination by microorganisms. In this context, the aim of this work was to develop biopolymeric fil...


Biodegradation of polybutylene adipate terephthalate and polyhydroxybutyrate/po...

Fernandes, Miguel ângelo Cavaleiro; Vicente, A. A.; Salvador, Andreia Filipa Ferreira

Recently, blends and composites of biodegradable polymers have been developed to replace petroleum-based plastics. Polybutylene adipate terephthalate and polyhydroxybutyrate are polymers that have received much attention due to their interesting mechanical properties. Polyhydroxybutyrate is highly biodegradable. However, polybutylene adipate terephthalate is quite difficult to biodegrade under environmental con...


The role of ohmic heating in tailoring pea protein functionality

Avelar, Zita Sofia Barbosa; Loureiro, Luís Carlos Sá; Leite, Ana Catarina Carvalho Oliveira; Vicente, A. A.; Rodrigues, Rui Miguel Martins

Plant-derived proteins have been growing in interest for the design of innovative foods and ingredients following the trend of animal protein substitution. These proteins display interesting functional properties, including emulsifying, foaming, and gelling capacity. Unfortunately, commercially available plant protein ingredients often present limited functionality due to the modifications induced during produc...


Effect of foliar biostimulant application on bioactive compounds and antioxidan...

Lopes, Tiago Alexandre Pedro; Silva, Ana Paula; Aires, Alfredo; Carvalho, Rosa; Ferreira, Maria; Vicente, A. A.; Gonçalves, Berta

Blueberries are rich in bioactive compounds and antioxidants whose contents can be significantly affected by pre-harvest agronomic practices. Thus, using natural biostimulants like Ecklonia maxima (EM) extract and glycine betaine (GB) is a promising strategy to improve blueberry quality. The effect of foliar pre-harvest application of EM and GB on bioactive compounds and antioxidant capacity (AC) of Duke and Dr...


Assessment of marine microalgae's bioactive extracts potential for food applica...

Sousa, Vítor Emanuel Silva; Coelho, M.; Martins, Joana T.; Pereira, Ricardo Nuno Correia; Maciel, Filipe; Pintado, M.; Dias, Oscar; Vicente, A. A.

The growing demand for sustainable and functional food ingredients has driven interest in marine microalgae as a source of bioactive compounds. This study evaluates the bioactivity, safety, and metabolite profile of extracts from Dunaliella salina and Pavlova gyrans, produced through two extraction methods: osmotic shock and bead milling with ethanol. Aqueous extracts were rich in peptides and amino acids, whil...


Assessment of marine microalgae's bioactive extracts potential for food applica...

Sousa, V.; Coelho, M.; Martins, J.; Pereira, R. N.; Maciel, F.; Pintado, M.; Dias, O.; Vicente, A. A.; Geada, P.

The growing demand for sustainable and functional food ingredients has driven interest in marine microalgae as a source of bioactive compounds. This study evaluates the bioactivity, safety, and metabolite profile of extracts from Dunaliella salina and Pavlova gyrans, produced through two extraction methods: osmotic shock and bead milling with ethanol. Aqueous extracts were rich in peptides and amino acids, whil...


In vitro digestion and cytotoxicity study of cannabidiol-loaded nanostructured ...

Vardanega, Renata; Lüdtke, Fernanda Luisa; Loureiro, Luís; Gonçalves, Raquel F. S.; Martins, Joana T.; Pinheiro, A. C.; Vicente, A. A.

Cannabidiol (CBD), a lipophilic compound with promising health benefits, such as analgesic, anti-inflammatory and neuroprotective effects, is characterized by low oral bioavailability, which limit its therapeutic application. To address these limitations, CBD was incorporated into nanostructured lipid carriers (NLCs), either as CBD-rich extract (CBDext) or as an isolate (CBDiso). The resulting systems NLC-CBDex...


Properties of bigels produced through WPI cold-set gelation and enriched with e...

Clímaco, Gabrielli Nunes; Gonçalves, Raquel Filipa Silva; Fernandes, Jean Michel; Pinheiro, A. C.; Vicente, A. A.; Fasolin, Luiz Henrique

The effect of incorporating Hibiscus sabdariffa L. leaf extract into bigels formulated with whey protein isolate (WPI), glycerol monostearate (GM), and soy lecithin was investigated. The hydrogel phase contained WPI (11% w/v) and NaCl (200 mmol/L), while the oleogel phase included sunflower oil, GM (10% w/v), and soy lecithin (5% w/v). Extracts obtained via supercritical fluid (SFE) and pressurized liquid (PLE)...


Toxicological profiling of nutraceutical-grade curcumin-loaded solid lipid nano...

Gonçalves, Raquel F. S.; Ribeiro, Joana; Martins, Joana T.; Passos, Marisa Sárria Pereira; Gomes, Andreia Castro; Vicente, A. A.; Pinheiro, Ana C.

The development of bio-based nanostructures to encapsulate bioactive compounds represents a promising approach for creating functional foods with improved nutritional and health benefits. However, their regular dietary use raises safety concerns, even when using food-grade ingredients. This study aimed to evaluate the toxicity of curcumin-loaded solid lipid nanoparticles (SLN_curc) through in vitro assays with ...


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