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Functional characterization of ShK domain-containing protein in the plant-paras...

Mendonça, Madalena; Vicente, Cláudia; Espada, Margarida

ShK domain-containing proteins are peptides found in different parasitic and venomous organisms. From a previous transcriptomic dataset from Bursaphelenchus xylophilus, a plant-endoparasitic nematode that infects forest tree species, we identified 96 transcripts potentially as ShK domain-containing proteins with unknown function in the nematode genome. This study aimed to characterize and explore the functional...


Guidelines for the reliable use of high throughput sequencing technologies to d...

Massart, Sebastien; Adams, Ian; Al Rwahnih, Maher; Boonham, Neil; Candresse, Thierry; Vicente, Cláudia

High-throughput sequencing (HTS) technologies have the potential to become one of the most signi cant advances in molecular diagnostics. Their use by researchers to detect and characterize plant pathogens and pests has been growing steadily for more than a decade and they are now envisioned as a routine diagnostic test to be deployed by plant pest diagnostics laboratories. Nevertheless, HTS technologies and dow...


The role of Pratylenchus penetrans transthyretin-like protein in parasitism.

Espada, Margarida; Vicente, Cláudia; Branco, Jordana; Mota, Manuel; Vieira, Paulo

Worldwide crop losses due to plant-parasitic nematodes have been estimated at $118 billion annually, with root lesion nematodes (RLN), Pratylenchus spp., ranking third in terms of economic losses. The economic impact of Pratylenchus spp. is due to their wide host range, comprising more than 400 plant species, including agronomically important crops, ornamentals/floral plants, and fruit trees. Surveys conducted ...


The fight against plant-parasitic nematodes: current status of bacterial and fu...

Pires, David; Vicente, Cláudia; Menéndez, Esther; Faria, Jorge; Rusinque, Leidy; Camacho, Maria João; Inácio, Lurdes

Plant-parasitic nematodes (PPNs) are among the most notorious and underrated threats to food security and plant health worldwide, compromising crop yields and causing billions of dollars of losses annually. Chemical control strategies rely heavily on synthetic chemical nematicides to reduce PPN population densities, but their use is being progressively restricted due to environmental and human health concerns, ...


Nematode-Mycobiota interactions in Pine Wilt Disease

Vicente, Cláudia; Faria, Jorge; Espada, Margarida; Nóbrega, Filomena; Bonifácio, Luís; Bragança, Helena; Sousa, Edmundo; Inácio, ML

Pine wilt disease is one of the most important diseases for conifer forests worldwide. This complex disease involves the interaction between three primary biological elements - the plant parasitic nematode Bursaphelenchus xylophilus; the insect-vector Monochamus sp., and the host tree Pinus spp. – and other secondary elements such as endophytic bacteria and fungi. The development of B. xylophilus is strongly as...


Effects of nematicidal phytochemicals on potato germination

Barbosa, Pedro; Vicente, Cláudia; Espada, Margarida; Figueiredo, AC; Mota, Manuel

Plant parasitic nematodes are responsible for significant productivity losses in the potato crop. Among them, the root lesion nematodes (RLN), ranks 3rd in terms of induced damage. Integrated in a wider study that aims to replace the common application of synthetic compounds for the control of potato nematodes by environment safe plant secondary metabolites, this study intended to evaluate potato seed germinati...


CENTR(AR): pulmões em andamento: manual de atividade física domiciliário

Marques, Alda (coord.); Simões, Adriana; Grave, Ana; Oliveira, Ana Carolina; Vieira, Ana; Laranjeiro, Ana Rita; Roque, André Gomes; Rijo, Ana Sofia

Sem resumo disponível.


Characterisation of transthyretin-like proteins of the plant-parasitic nematode...

Espada, Margarida; Vicente, Cláudia; Branco, Jordana; Mota, Manuel; Vieira, Paulo

Transthyretin-like proteins (TTL) belong to a widely conserved family present only in the Phylum Nematoda. Although several TTLs (protein domain PF01060) have been identified for both animal and plant-parasitic nematodes most of their function(s) remains unclear. In Pratylenchus penetrans, a migratory plant-parasitic nematode, transcriptomic data revealed that some members of this family are abundantly expresse...


Nematicidal actions of the marigold exudate α-terthienyl: oxidative stress-indu...

Hamaguchi, Takahiro; Sato, Kazuki; Vicente, Cláudia; Hasegawa, Koichi

α-terthienyl is an allelochemical derived from the roots of marigold (Tagetes spp.), which is used to suppress plant parasitic nematodes. We investigated the nematicidal activity of α-terthienyl against the model organism Caenorhabditis elegans and the root-knot nematode, Meloidogyne incognita. As reported previously, α-terthienyl action was much higher after photoactivation, but was still effective against C. ...


Does the dilution agent affect root lesion nematode mortality evaluation?

Barbosa, Pedro; Vicente, Cláudia; Figueiredo, Ana Cristina; Mota, Manuel

Phytochemicals are known for their profuse and well documented biological and pharmaceutical capabilities. They have been extensively tested in nematode, insect or bacteria mortality bioassays. Since some of these compounds have a very low solubility in water, choosing the right dilution agent or technique is of the utmost importance. The selected agent should allow a dilution that does not significantly alter ...


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