17 documents found, page 1 of 2
Influence of Choline Chloride on the Phase Equilibria and Partition Performance...
Alves, Mariana B.; Lopes, André M.; Santos, Nathalia A.; Santos-Ebinuma, Valéria C.; Vicente, Filipa A.; Pereira, Jorge F. B.A series of polymeric aqueous biphasic systems (ABS) were determined using polyethylene glycol (PEG) and sodium polyacrylate (NaPA) with choline chloride ([Ch]Cl) as an adjuvant. The effect of (i) PEG and NaPA molecular weights, (ii) PEG functionalization, (iii) [Ch]Cl addition (at different concentrations), and (iv) temperature (25, 37 and 50 C) was evaluated through their ability to promote the two-phase sepa...
Purification of immunoglobulin Y from egg yolk using thermoresponsive aqueous m...
Vicente, Filipa A.; Castro, Leonor S.; Mondal, Dibyendu; Coutinho, João A. P.; Tavares, Ana P. M.; Ventura, Sónia P. M.; Freire, Mara G.Immunoglobulin Y (IgY) represents an important class of antibodies, being present in egg yolk, with relevant medical applications and involving non-invasive methods of extraction. However, due to the complexity of egg yolk, the purification levels required for use in most medical applications demands the application of multi-step and cost-intensive techniques. Therefore, it is of upmost importance to develop a ...
Crustacean waste biorefinery as a sustainable cost-effective business model
Vicente, Filipa A.; Ventura, Sónia P. M.; Passos, Helena; Dias, Ana C. R. V.; Torres-Acosta, Mario A.; Novak, Uroš; Likozar, BlažMarine-derived food wastes mainly include seafood, fish and feed production resources. From the crustaceans traded annually, 6 to 8 million tonnes of valuable shrimp, lobster and/or crab shells waste are produced worldwide. In this systemic work, the researchers with complementary technical expertise, covering the fields of chemical engineering design, chemistry, materials, predictive environmental sciences and...
Development of a microfluidic platform for R-Phycoerythrin purification using a...
Seručnik, Mojca; Vicente, Filipa A.; Brečko, Živa; Coutinho, João A. P.; Ventura, Sónia P. M.; Žnidaršič-Plazl, PolonaTemperature-dependent aqueous micellar two-phase systems (AMTPSs) have recently been gaining attention in the isolation of high-added-value biomolecules from their natural sources. Despite their sustainability, aqueous two-phase systems, and particularly AMTPSs, have not been extensively applied in the industry, which might be changed by applying process integration and continuous manufacturing. Here, we report...
Unravelling the interactions between surface-active ionic liquids and triblock ...
Pérez-Sánchez, Germán; Vicente, Filipa A.; Schaeffer, Nicolas; Cardoso, Inês S.; Ventura, Sónia P. M.; Jorge, Miguel; Coutinho, João A. P.The tunable properties of surface-active ionic liquids (SAILs) and Pluronics are dramatically magnified by combining them in aqueous solutions. The thermo-controlled character of both, essential in the extraction of valuable compounds, can be fine-tuned by properly selecting the Pluronic and SAIL nature. However, further understanding of the nanoscale interactions directing the aggregation in these complex mixt...
Separation and purification of biomacromolecules based on microfluidics
Vicente, Filipa A.; Plazl, Igor; Ventura, Sónia P. M.; Žnidaršič-Plazl, PolonaSeparation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and re...
Use of ionic liquids as cosurfactants in mixed aqueous micellar two-phase syste...
Vicente, Filipa A.; Bairos, Jéssica; Roque, Manuel; Coutinho, João A. P.; Ventura, Sónia P. M.; Freire, Mara G.A large fraction of unused clinical transfusion plasma is nowadays discarded. Nevertheless, some proteins, including serum albumin, immunoglobulins, and clot factors, are still viable for use in diagnosis or research. However, lasma is a complex matrix, requiring multiple steps and timeconsuming methods for the isolation of proteins. In this work, aqueous micellar two-phase systems (AMTPS) composed of nonionic ...
R-phycoerythrin extraction and purification from fresh Gracilaria sp. using the...
Vicente, Filipa A.; Cardoso, Inês S.; Martins, Margarida; Gonçalves, Cátia V. M.; Dias, Ana C. R. V.; Domingues, Pedro; Coutinho, João A. P.R-phycoerythrin is a high added-value protein found in red macroalgae with several interesting properties. Despite the promising results found when R-phycoerythrin is used as an optically active center in luminescent solar concentrators (LSCs), it still has some problems that can be attributed to the low stability of the R-phycoerythrin in the presence of the specific contaminant proteins found in the crude ext...
Integration of aqueous (micellar) two-phase systems on the proteins separation
Vicente, Filipa A.; Santos, João H. P. M.; Pereira, Inês M. M.; Gonçalves, Cátia V. M.; Dias, Ana C. R. V.; Coutinho, João A. P.; Ventura, Sónia P. M.A two-step approach combining an aqueous two-phase system (ATPS) and an aqueous micellar two-phase system (AMTPS), both based on the thermo-responsive copolymer Pluronic L-35, is here proposed for the purification of proteins and tested on the sequential separation of three model proteins, cytochrome c, ovalbumin and azocasein. Phase diagrams were established for the ATPS, as well as co-existence curves for the...
Rationalizing the phase behavior of triblock copolymers through experiments and...
Pérez-Sánchez, Germán; Vicente, Filipa A.; Schaeffer, Nicolas; Cardoso, Inês S.; Ventura, Sónia P. M.; Jorge, Miguel; Coutinho, João A. P.In this paper, we develop a new coarse-grained model, under the MARTINI framework, for Pluronic block copolymers that is able to describe the self-assembly mechanism and reproduce experimental micelle sizes and shapes. Previous MARTINI-type Pluronic models were unable to produce realistic micelles in aqueous solution, and thus our model represents a marked improvement over existing approaches. We then applied t...