83 documents found, page 1 of 9
Single-cell liquid-core microcapsules for biomedical applications
Pires-Santos, Manuel; Carreira, Mariana; Morais, Bruno P.; Perfeito, Francisca G.; Oliveira, Mariana B.; Monteiro, Cátia F.; Nadine, Sara; Mano, João F.More recently, single-cell encapsulation emerged as a promising field in biomedicine due to its potential applications, in cell analysis and therapy. Traditional techniques involve embedding cells in crosslinked polymers to create continuous microgels, suitable mainly for adherent cells, or encapsulating them in droplets for only short-term analysis, due to their instability. In this study, we developed a metho...
Shape-versatile fixed cellular materials for multiple target immunomodulation
Sousa, Ana Rita; Cunha, Ana F.; Santos-Coquillat, Ana; Estrada, Beatriz Hernaez; Spiller, Kara L.; Barão, Marta; Rodrigues, Artur Filipe; Simões, SusanaTherapeutic cells are usually administered as living agents, despite the risks of undesired cell migration and acquisition of unpredictable phenotypes. Additionally, most cell-based therapies rely on the administration of single cells, often associated with rapid in vivo clearance. Three-dimensional cellular materials may be useful to prolong the effect of cellular therapies and offer the possibility of creatin...
Natural Polymer-Polyphenol Bioadhesive Coacervate with Stable Wet Adhesion, Ant...
Sacramento, Margarida M. A.; Oliveira, Mariana B.; Gomes, José R. B.; Borges, João; Freedman, Benjamin R.; Mooney, David J.; Rodrigues, João M. M.Medical adhesives are emerging as an important clinical tool as adjuvants for sutures and staples in wound closure and healing and in the achievement of hemostasis. However, clinical adhesives combining cytocompatibility, as well as strong and stable adhesion in physiological conditions, are still in demand. Herein, a mussel-inspired strategy is explored to produce adhesive coacervates using tannic acid (TA) an...
Spontaneous formation of solid shell polymeric multicompartments at all-aqueous...
Perfeito, Francisca G.; Vilabril, Sara; Cerqueira, Andreia; Oliveira, Mariana B.; Mano, João F.Multicompartmental capsules have demonstrated value in fields ranging from drug release, mimetics of artificial cells, to energy conversion and storage. However, the fabrication of devices with different compartments usually requires the use of toxic solvents, and/or the adaptation of technically demanding methods, including precision microfluidics and multistep processes. The spontaneous formation of multi-cor...
Exploring the potential of all-aqueous immiscible systems for preparing complex...
Gonçalves, Raquel C.; Oliveira, Mariana B.; Mano, João F.All-aqueous immiscible systems derived from liquid-liquid phase separation of incompatible hydrophilic agents such as polymers and salts have found increasing interest in the biomedical and tissue engineering fields for the last years. The unique characteristics of aqueous interfaces, namely their low interfacial tension and elevated permeability, as well as the non-toxic environment and high-water content of t...
Reversible imine crosslinking in waterborne self-healing polymer coatings
Martins, Tiago D.; Viciosa, M. Teresa; Oliveira, Mariana B.; Fernandes, Auguste; Mano, João F.; Baleizão, Carlos; Farinha, José Paulo S.Waterborne polymer coatings have the potential to address the environmental concerns associated with solvent based systems. To improve their performance without using volatile organic compounds, we propose a new approach based on reconfigurable covalent crosslinking that provides mechanical resistance and self-healing properties. The new waterborne polymer coatings are based on mixtures of aldehyde- and amine-f...
All-Aqueous freeform fabrication of perfusable self-standing soft compartments
Gonçalves, Raquel C.; Vilabril, Sara; Neves, Catarina M. S. S.; Freire, Mara G.; Coutinho, João A. P.; Oliveira, Mariana B.; Mano, João F.Compartmentalized structures obtained in all-aqueous settings have shown promising properties as cell encapsulation devices, as well as reactors for trans-membrane chemical reactions. While most approaches focus on the preparation of spherical devices, advances on the production of complex architectures have been enabled by the interfacial stability conferred by emulsion systems, namely mild aqueous two-phase s...
Tricalcium phosphate doped with Mg2+ and combinations of Mn2+, Zn2+ and Fe3+: A...
Macedo, Duarte F.; Cunha, Ana F.; Mano, João F.; Oliveira, Mariana B.; Silva, Abílio P.Bioactive ceramics such as tricalcium phosphate (TCP) doped with metal ions are often used to replace natural bone in orthopedic and dental function, both in structural and coating applications. Although the addition of different ions to TCP has been correlated with improved mechanical performance, as well as pro-osteogenic and osteointegrative biological activities, the effect of combining different ions in si...
Cell response in free-packed granular systems
Cunha, Ana F.; Matias, André F. V.; Dias, Cristóvão S.; Oliveira, Mariana B.; Araújo, Nuno A. M.; Mano, João F.The study of the interactions of living adherent cells with mechanically stable (visco)elastic materials enables understanding and exploitation of physiological phenomena mediated by cell-extracellular communication. Insights into the interaction of cells and surrounding objects with different stability patterns upon cell contact might unveil biological responses to engineer innovative applications. Here, we hy...
NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-der...
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Correia, Marlene; Ferreira, Filipa; Bonifazio, Giulia; Goodfellow, Brian J.; Oliveira, Mariana B.This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics...