The aim of this work was to determine the viability of spermatocrit use in Piabanha's sperm concentration estimation. Gametogenesis of piabanha Brycon insignis (n=29) was induced with crude pituitary extract of carp and semen was further collected. The spermatocrit was determined using cappilar tube micro-centrifuge. The sperm concentration was verified by counting in "Neubauer chamber", after dilution. The ver...