17 documents found, page 1 of 2
Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae sero...
Santos, Lucas Fernando dos; Polveiro, Richard Costa; Moreira, Thalita Scatamburlo; Vidigal, Pedro Marcus Pereira; Chang, Yung-FuThe bacterium Actinobacillus pleuropneumoniae is the etiological agent of Contagious Porcine Pleuropneumonia, a disease responsible for economic losses in the swine industry worldwide. A. pleuropneumoniae is capable of producing proteinaceous exotoxins responsible for inducing hemorrhagic lesions, one of which is ApxI. Few studies have conducted an in-depth evaluation of polymorphisms of the nucleotides that ma...
Screening and characterization of prophages in Desulfovibrio genomes
Crispim, Josicelli Souza; Dias, Roberto Sousa; Vidigal, Pedro Marcus Pereira; Sousa, Maíra Paula de; Silva, Cynthia Canêdo da; Santana, Mateus FerreiraBacteria of the genus Desulfovibrio belong to the group of Sulphate Reducing Bacteria (SRB). SRB generate significant liabilities in the petroleum industry, mainly due to their ability to microbiologically induce corrosion, biofilm formation and H2S production. Bacteriophages are an alternative control method for SRB, whose information for this group of bacteria however, is scarce. The present study developed a...
Desulfovibrio alaskensis prophages and their possible involvement in the horizo...
Crispim, Josicelli Souza; Dias, Roberto Sousa; Laguardia, Clara Nogueira; Araújo, Larissa Cristina; Silva, Jéssica Duarte daDesulfovibrio alaskensis is a Gram-negative bacterial species that belongs to the group of Sulphate Reducing Bacteria (SRB) and presents prophages in genomes, a common characteristic of the genus Desulfovibrio. Genetic material can be transported by outer membrane vesicles, however, no data regarding the production of these vesicles has been reported for D. alaskensis. To verify the expression of D. alaskensis ...
Retrospective detection and genetic characterization of Porcine circovirus 3 (p...
Saraiva, Giuliana Loreto; Vidigal, Pedro Marcus Pereira; Assao, Viviane Sisdelli; Fajardo, Murilo Leone Miranda; Loreto, Alerrandra Nunes SaraivaPorcine circovirus 3 (PCV3) is an emerging virus that was first identified in the United States in 2016. Since its first detection, PCV3 has already been found in America, Asia, and Europe. Although PCV3 has already been described in Brazil, knowledge of its detection and sequence variation before 2016 is limited, as well as its distribution in the main swine producing regions of Brazil. In this study, 67 porci...
Genomic and biological characterization of a new member of the genus Phikmvviru...
Xavier, André da Silva; Silva, Fernanda Pereira da; Vidigal, Pedro Marcus Pereira; Lima, Thamylles Thuany Mayrink; Souza, Flavia Oliveira deBacterial wilt caused by Ralstonia spp., soil-borne Gram-negative bacteria, is considered one of the most important plant diseases in tropical and subtropical regions of the world. A large number of bacteriophages capable of lysing or physiologically reprogramming cells of Ralstonia spp. have been reported in Asia. Despite the potential use of these organisms in the management of bacterial wilt, information on ...
A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substit...
Salgado, Rafael Locatelli; Vidigal, Pedro Marcus Pereira; Gonzaga, Natalia F.; Souza, Luiz F. L. de; Polêto, Marcelo D.; Onofre, Thiago SouzaPorcine circovirus-2 (PCV2) is the etiologic agent of several diseases in pigs, including multi-systemic wasting syndrome (PMWS). In this work, a new mutant PCV2b was isolated from PMWS-affected pigs on a Brazilian farm. Its genome showed high sequence similarity (>99 % identity) to those from a group of emerging mutants isolated from cases of PMWS outbreaks in vaccinated pigs in China, the USA and South Korea....
Transcriptome analysis of the thermotolerant yeast Kluyveromyces marxianus CCT ...
Diniz, Raphael Hermano Santos; Villada, Juan C.; Alvim, Mariana Caroline Tocantins; Vidigal, Pedro Marcus Pereira; Vieira, Nívea MoreiraThe thermotolerant yeast Kluyveromyces marxianus displays a potential to be used for ethanol production from both whey and lignocellulosic biomass at elevated temperatures, which is highly alluring to reduce the cost of the bioprocess. Nevertheless, contrary to Saccharomyces cerevisiae, K. marxianus cannot tolerate high ethanol concentrations. We report the transcriptional profile alterations in K. marxianus un...
Distribution of infectious bronchitis virus strains in different organs and evi...
Pereira, Claiton Gonçalves; Saraiva, Giuliana Loreto; Vidigal, Pedro Marcus Pereira; Fietto, Juliana Lopes Rangel; Bressan, Gustavo CostaOn the basis of partial sequencing of the infectious bronchitis virus (IBV) S1 gene, this study investigated the molecular diversity of the virus in two life periods of a batch of breeding hens at the field level. The chicks were vaccinated against IBV on the second day of life with the vaccine Ma5, but at the age of 18 days, they exhibited clinical signs and macroscopic lesions compatible with avian infectious...
An integrative overview of the molecular and physiological responses of sugarca...
Vital, Camilo Elber; Giordano, Andrea; Soares, Eduardo de Almeida; Williams, Thomas Christopher Rhys; Mesquita, Rosilene OliveiraDrought is the main abiotic stress constraining sugarcane production. However, our limited understanding of the molecular mechanisms involved in the drought stress responses of sugarcane impairs the development of new technologies to increase sugarcane drought tolerance. Here, an integrated approach was performed to reveal the molecular and physiological changes in two closely related sugarcane cultivars, inclu...
Genetic evaluation of IS900 partial sequence of Mycobacterium avium subsp. para...
Carvalho, Isabel Azevedo; Silva, Victor Oliveira; Vidigal, Pedro Marcus Pereira; Silva Junior, Abelardo; Moreira, Maria Aparecida ScatamburloMycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis. Insertion sequence IS900 is used for the identification of MAP. The objective of this study was to verify the genetic conservation of IS900 sequences in raw milk samples. To evaluate genetic conservation, 206 quarter milk samples and 16 bulk-tank milk samples were collected. DNA extraction and IS900 PCR were performed ...