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Substrate-dependent oxidative inactivation of a W-dependent formate dehydrogena...
Vilela-Alves, Guilherme; Manuel, Rita R.; Viegas, Aldino; Carpentier, Philippe; Biaso, Frédéric; Guigliarelli, Bruno; Pereira, Inês A.C.Funding Information: This work was financially supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia (FCT, Portugal) through fellowship no. 2023.00286.BD (G. V.-A.) and DFA/BD/7897/2020 (R. R. M.), grant no. PTDC/BII-BBF/2050/2020 (http://doi.org/10.54499/PTDC/BII-BBF/2050/2020) (I. A. C. P. and M. J. R.), research contract 2020.00043.CEECIND (A. V.) and R&D units MOSTMICRO-ITQB (grant no. UIDB/04612...
Biophysical and pharmacokinetic characterization of a small-molecule inhibitor ...
Gopalswamy, Mohanraj; Kroeger, Tobias; Bickel, David; Frieg, Benedikt; Akter, Shahina; Schott-Verdugo, Stephan; Viegas, Aldino; Pauly, ThomasFunding Information: This work was supported by a grant by the state of North-Rhine Westphalia and the European Fonds for Regional Development EFRE.NRW 2014-2020 to HG. We are grateful for computational support and infrastructure provided by the “Zentrum für Informations- und Medientechnologie” (ZIM) at the Heinrich Heine University Düsseldorf and the computing time provided by the John von Neumann Institute fo...
The architecture of the 10-23 DNAzyme and its implications for DNA-mediated cat...
Borggräfe, Jan; Gertzen, Christoph G. W.; Viegas, Aldino; Gohlke, Holger; Etzkorn, ManuelPublisher Copyright: © 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.; Understanding the molecular features of catalytically active DNA sequences, so-called DNAzymes, is essential not only for our understanding of the fundamental properties of catalytic nucleic acids in general, but may well be the key to unravelling their full po...
A dual cohesin-dockerin complex binding mode in Bacteroides cellulosolvens cont...
Duarte, Marlene; Viegas, Aldino; Alves, Victor D.; Prates, José A. M.; Ferreira, Luís M. A.; Najmudin, Shabir; Cabrita, Eurico J.; Carvalho, Ana LuísaThe Cellulosome is an intricate macromolecular protein complex that centralizes the cellulolytic efforts of many anaerobic microorganisms through the promotion of enzyme synergy and protein stability. The assembly of numerous carbohydrate processing enzymes into a macromolecular multiprotein structure results from the interaction of enzyme-borne dockerin modules with repeated cohesin modules present in noncatal...
Structural and dynamic insights revealing how lipase binding domain MD1 of Pseu...
Viegas, Aldino; Dollinger, Peter; Verma, Neha; Kubiak, Jakub; Viennet, Thibault; Seidel, Claus A. M.; Gohlke, Holger; Etzkorn, Manuel; Kovacic, FilipID: HDD16, project IDs: 11237 ET 103/2-1 to ME; no. SE 1195/16-1 no. GO 1367/1-1 JA 448/8-1 ET 103/2-1 PINFRA/22161/2016 -1; Folding and cellular localization of many proteins of Gram-negative bacteria rely on a network of chaperones and secretion systems. Among them is the lipase-specific foldase Lif, a membrane-bound steric chaperone that tightly binds (KD = 29 nM) and mediates folding of the lipase LipA, a v...
Diverse specificity of cellulosome attachment to the bacterial cell surface
Brás, Joana L. A.; Pinheiro, Benedita A.; Cameron, Kate; Cuskin, Fiona; Viegas, Aldino; Najmudin, Shabir; Bule, Pedro; Pires, Virginia M. R.This work was supported by the EU FP7 programme under the WallTraC project (grant No. 263916) and by projects PTDC/BIA-MIC/5947/2014, RECI/BBB-BEP/0124/2012 and EXPL/BIA-MIC/1176/2012 supported by Fundacao para a Ciencia e Tecnologia (FCT-MCTES). The Research Unit UCIBIO (Unidade de Ciencias Biomoleculares Aplicadas) is financed by national funds from FCT/MCTES EC (UID/Multi/04378/2013) and co-financed by the E...
Is prnt a pseudogene? identification of ram prt in testis and ejaculated sperma...
Pimenta, Jorge; Domingos, Ana; Santos, P.; Marques, Carla C.; Cantante, Cátia; Santos, Ana; Barbas, João P.; Baptista, Maria C.; Horta, António E.M.A hallmark of prion diseases or transmissible spongiform encephalopaties is the conversion of the cellular prion protein (PrPC), expressed by the prion gene (prnp), into an abnormally folded isoform (PrPSc) with amyloid-like features that causes scrapie in sheep among other diseases. prnp together with prnd (which encodes a prion-like protein designated as Doppel), and prnt (that encodes the prion protein testi...