6 documents found, page 1 of 1
Raman spectroscopy for classification of neoplastic and non-neoplastic CAM colo...
Esteves, Bruno Miguel Carpinteiro; Pimenta, Sara Filomena Ribeiro; Maciel, Marino Jesus Correia; Costa, Marta Sílvia Freitas; Baltazar, FátimaThis paper demonstrates the potential of Raman spectroscopy for differentiating neoplastic from non-neoplastic colon tumors, obtained with the CAM (chicken chorioallantoic membrane) model. For the CAM model two human cell lines were used to generate two types of tumors, the RKO cell line for neoplastic colon tumors and the NCM460 cell line for non-neoplastic colon tumors. The Raman spectra were acquired with a ...
The chick chorioallantoic membrane assay as an in vivo model for colon tumor an...
Pereira, Hugo Martins; Freitas, Ruben Brandão; Maciel, Marino Jesus Correia; Pimenta, Sara Filomena Ribeiro; Costa, Marta Sílvia FreitasOptical coherence tomography (OCT) has emerged as a promising imaging modality for biomedical research. In this paper, a commercial OCT system was used to differentiate two colon cell lines implanted in a chick embryo chorioallantoic membrane (CAM) model, using the attenuation coefficient of light. Malignant and non-malignant tumors were developed from RKO and NCM460 cell lines, respectively. OCT B-scan images ...
Prognostic value of Monocarboxylate Transporter 1 overexpression in cancer: a s...
Silva, Ana; Cerqueira, Mónica Costa; Rosa, Beatriz; Sobral, Catarina; Pinto-Ribeiro, Filipa; Costa, Marta Sílvia Freitas; Baltazar, FátimaEnergy production by cancer is driven by accelerated glycolysis, independently of oxygen levels, which results in increased lactate production. Lactate is shuttled to and from cancer cells via monocarboxylate transporters (MCTs). MCT1 works both as an importer and an extruder of lactate, being widely studied in recent years and generally associated with a cancer aggressiveness phenotype. The aim of this systema...
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards re...
Freitas, Ana Sofia; Costa, Marta Sílvia Freitas; Pontes, Olívia; Seidel, Veronique; Proença, M. Fernanda R. P.; Cardoso, Susana M.Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b...
Exploitation of new chalcones and 4H-chromenes as agents for cancer treatment
Pontes, Olívia; Costa, Marta Sílvia Freitas; Santos, Filipa; Marques, Maria Belém Sousa Sampaio; Dias, Tatiana Alexandra Freitas PerdigãoChalcone and chromene derivatives were synthesized in good yield through simple and effective reactions using innocuous solvents such as water and ethanol and high yielding aldol condensations. Generally, the reactions were performed at room temperature, leading to the isolation of highly pure compounds. These compounds were tested on breast cancer cells (MCF-7 and Hs578T) and breast non-neoplastic cells (MCF-1...
Síntese de derivados de cromeno para screening como agentes antipsicóticos
Costa, Marta Sílvia FreitasO plano de trabalhos que orientou o tema de doutoramento tinha por objectivo o desenvolvimento de métodos experimentais para a síntese de cromenos e derivados, principalmente a partir da reacção de salicilaldeídos com compostos de metileno activado, como potenciais agentes antipsicóticos. Foram sintetizados diversos compostos de metileno activado, na sua maioria utilizando métodos simples que levaram a bons ren...