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PratyOmics - Metabolómica da planta para o controlo do nemátode das lesões radi...

Barbosa, Pedro; Costa, Marina; Espada, Margarida; Teixeira, Dora; Brás, Teresa; Duarte, Fátima; Figueiredo, Joana; Cardoso, Joana; Esteves, Ivânia

O nemátode-das-lesões-radiculares (NLR), Pratylenchus penetrans, é um endoparasita migratório que afeta culturas agrícolas economicamente importantes, como a batata, estando atualmente presente na lista de pragas reguladas não sujeitas a quarentena A2 da União Europeia (UE 2016/2031). Em alguns países europeus, como Portugal, esta espécie foi detetada em campos de produção de batata estando relacionada com o se...


How parasitism genes are regulated: a motif to search for genes regulators in t...

Mendonça, Madalena; Vicente, Cláudia S. L.; Barbosa, Pedro; Velada, Isabel; Vieira, Paulo; Faria, Jorge; Santos, Margarida; Nóbrega, Filomena

Plant-parasitic nematodes threaten global agricultural and forestry systems. The search for new control strategies in line with the EU’s sustainability goals highlight significant knowledge gaps. Like all other plant pathogens, plant-parasitic nematodes deliver several parasitism proteins (effectors) into the host plant to cause disease. NemaWAARS project focuses on mechanism(s) of regulation and gene control e...


The root lesion nematode effector Ppen10370 is essential for parasitism of Prat...

Vieira, Paulo; Vicente, Claudia; Branco, Jordana; Buchan, Gary; Mota, Manuel; Nemchinov, Lev

The root lesion nematode, Pratylenchus penetrans, is a migratory species that attacks a broad range of crops. Like other plant pathogens, P. penetrans deploys a battery of secreted protein effectors to manipulate plant hosts and induce disease. Although several candidate effectors of P. penetrans have been identified, detailed mechanisms of their functions and particularly their host targets remain largely unex...


The role of Pratylenchus penetrans transthyretin-like protein in parasitism.

Espada, Margarida; Vicente, Cláudia; Branco, Jordana; Mota, Manuel; Vieira, Paulo

Worldwide crop losses due to plant-parasitic nematodes have been estimated at $118 billion annually, with root lesion nematodes (RLN), Pratylenchus spp., ranking third in terms of economic losses. The economic impact of Pratylenchus spp. is due to their wide host range, comprising more than 400 plant species, including agronomically important crops, ornamentals/floral plants, and fruit trees. Surveys conducted ...


Phytochemicals as Biopesticides against the Pinewood Nematode Bursaphelenchus x...

Faria, Jorge MS; Barbosa, Pedro; Vieira, Paulo; Vicente, CSL; Figueiredo, AC; Mota, Manuel

The impacts of a rapidly changing environment together with the growth in global trade activities has promoted new plant pest pandemic events in forest ecosystems. The pinewood nematode (PWN), Bursaphelenchus xylophilus, causes strong worldwide economic and ecological impacts. Direct control is performed through trunk injection of powerful nematicides, however many of these (hemi)synthetic compounds have raised...


O NEMÁTODE‑DAS‑LESÕES‑RADICULARES, PRATYLENCHUS PENETRANS

Vicente, Cláudia S. L.; Inacio, Maria de Lurdes; Mota, Manuel; Vieira, Paulo

O nemátode-das-lesões-radiculares, Pratylenchus penetrans, é um dos nemátodes parasitas de plantas mais devastador a nível mundial, e uma ameaça para um vasto número de culturas de interesse económico. Em Portugal, é frequentemente detetado em campos de batata. A deteção atempada deste parasita no solo, aliada a boas práticas agrícolas, são fatores decisivos para a proteção das culturas.


Plant Metabolomics for the control of the root-lesion nematode Pratylenchus pen...

Vicente, Claudia; Faria, Jorge; Teixeira, Dora; Vieira, Paulo; Espada, Margarida; Barbosa, Pedro; Figueiredo, Joana; Cardoso, Joana; Esteves, Ivania

Pratylenchus penetrans, one of the most detrimental root-lesion nematode species, greatly reduces the production in numerous important agronomic crops (e.g., corn, potato), ornamental plants (e.g., lily, roses) and fruit trees (e.g., almond, cherry orchards). In the EU, P. penetrans has been reported as the most damaging species associated with potato (Solanum tuberosum L.). In Portugal, this species was also d...


Commercial Potato Cultivars Exhibit Distinct Susceptibility to the Root Lesion ...

Figueiredo, Joana; Vieira, Paulo; Abrantes, Isabel; Esteves, Ivânia

The root lesion nematode Pratylenchus penetrans is an important plant-parasitic nematode of potato. In this study, the susceptibility of commercial potato cultivars to P. penetrans was assessed. Nematode penetration was evaluated in cultivars Agria, Camel, Kennebec, Laura, Royata, and Stemster at 1, 3, 7, and 15 days after inoculation (DAI) with 750 nematodes/plant, and an egression assay at 3 DAI with 1000 nem...


Characterisation of transthyretin-like proteins of the plant-parasitic nematode...

Espada, Margarida; Vicente, Cláudia; Branco, Jordana; Mota, Manuel; Vieira, Paulo

Transthyretin-like proteins (TTL) belong to a widely conserved family present only in the Phylum Nematoda. Although several TTLs (protein domain PF01060) have been identified for both animal and plant-parasitic nematodes most of their function(s) remains unclear. In Pratylenchus penetrans, a migratory plant-parasitic nematode, transcriptomic data revealed that some members of this family are abundantly expresse...


Understanding the role and delivery of Pratylenchus penetrans effectors proteins

Vicente, Claudia; Espada, Margarida; Mota, Manuel; Vieira, Paulo

Considered one of the most damaging species of root lesion nematodes (RLN), the plant parasitic nematode (PPN) Pratylenchus penetrans is a serious issue worldwide in crop protection and productivity of important agronomic and horticultural crops (e.g. potato, corn). As other PPN, P. penetrans secrete a repertoire of effectors, which once delivered either in the apoplast or inside cells, can trigger and manipula...


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