4 documents found, page 1 of 1
Cells isolated from regenerating caudal fin of Sparus aurata can differentiate ...
Vijayakumar, Parameswaran; Cardeira, João; Laizé, Vincent; J. Gavaia, Paulo; Cancela, M. LeonorTeleosts have the ability to regenerate their caudal fin upon amputation. A highly proliferative mass of undifferentiated cells called blastema forms beneath wound epidermis and differentiates to regenerate all missing parts of the fin. To date, the origin and fate of the blastema is not completely understood. However, current hypotheses suggest that the blastema is comprised of lineage-restricted dedifferentia...
Isolation, culture, and differentiation of Blastema cells from the regenerating...
Vijayakumar, Parameswaran; Cancela, M. Leonor; Laizé, VincentThe caudal fin of teleost fish has become an excellent system for investigating the mechanisms of epimorphic regeneration. Upon amputation of the caudal fin, a mass of undi erentiated cells, called blastema, proliferate beneath the wound-epidermis and di erentiate into various cell types to faithfully restore the missing fin structures. Here we describe a protocol that can be used to isolate and culture blastem...
Central role of betaine-homocysteine S-methyltransferase 3 in chondral ossifica...
Rosa, Joana; Tiago, Daniel; Marques, Cátia L.; Vijayakumar, Parameswaran; Fonseca, Luís; Cancela, Leonor; Laizé, VincentBackground: To better understand the complex mechanisms of bone formation it is fundamental that genes central to signaling/regulatory pathways and matrix formation are identified. Cell systems were used to analyze genes differentially expressed during extracellular matrix mineralization and bhmt3, coding for a betaine-homocysteine S-methyltransferase, was shown to be down-regulated in mineralizing gilthead sea...
Development of an in vitro cell system from zebrafish suitable to study bone ce...
Vijayakumar, Parameswaran; Laizé, Vincent; Cardeira Da Silva, João; Trindade, Marlene; Cancela, M. LeonorMechanisms of bone formation and skeletal development have been successfully investigated in zebrafish using a variety of in vivo approaches, but in vitro studies have been hindered due to a lack of homologous cell lines capable of producing an extracellular matrix (ECM) suitable for mineral deposition. Here we describe the development and characterization of a new cell line termed ZFB1, derived from zebrafish ...