11 documents found, page 1 of 2
The impact of hepatitis E infection on hepatic fibrosis in liver transplanted p...
de Moraes,Adriano Claudio Pereira; Gouvea,Michele Gomes; Ferreira,Ariana Carolina; Pinho,João Renato Rebello; de Mello,Evandro SobrozaABSTRACT Hepatitis E Virus (HEV) is an infection known worldwide for its asymptomatic and self-limited course in most cases. Some cases progressing to chronicity have been described in immunosuppressed patients, especially in recipients of solid organ transplants. We evaluated laboratory parameters of HEV infection (HEV RNA, anti-HEV IgM and anti-HEV IgG) through enzyme-linked immunosorbent assay (Elisa), confi...
Long term persistence of coronavirus SARS-CoV-2 infection
Pinho,João Renato Rebello; Oliveira,Ketti Gleyzer de; Sitnik,Roberta; Maluf,Maira Marranghello; Rodrigues,Pedro Henrique SebeABSTRACT During the COVID-19 pandemic, a case of a long-term persistence of SARS-CoV-2 infection (from March 26 to May 20, 2020) was identified at a private hospital in São Paulo, SP, Brazil. The long-term positivity for SARS-CoV-2 in reverse transcriptase polymerase chain reaction tests of a patient diagnosed with COVID-19 suggests, at least part of patients who recovered, may still carry and transmit the SARS...
Hepatitis B viremia in HIV-coinfected individuals under antiretroviral therapy
Weissmann,Leonardo; Picone,Camila de Melo; Gouvêa,Michele Soares Gomes; Ferreira,Paulo Roberto Abrão; Viana,Mônica Salum Valverde BorsoiABSTRACT Background: Antiretroviral therapy (ART) has decreased AIDS incidence and mortality, rendering comorbidities, such as hepatitis B more relevant for people living with human immunodeficiency virus (HIV). Since antiretroviral drugs may also inhibit hepatitis B virus (HBV) replication, analyzing the impact of ART on management of hepatitis B in this population is important. Objective: To assess HBV viremi...
Distribution of HBV subgenotypes in Ribeirão Preto, Southeastern Brazil: a regi...
Chachá,Silvana Gama Florencio; Gomes-Gouvêa,Michele Soares; Malta,Fernanda de Mello; Ferreira,Sandro da Costa; Villanova,Márcia GuimarãesAbstract Hepatitis B virus (HBV) is distributed worldwide, with geographical variations regarding prevalence of the different genotypes. The aim of this study was to determine the HBV genotypes and subgenotypes circulating in Southeast Brazil and compare the genetic sequences found with HBV sequences previously described in the world. Sequences from 166 chronic HBV carriers were analyzed using the fragment cons...
Hepatitis C among blood donors: cascade of care and predictors of loss to follo...
Machado,Soraia Mafra; Almeida Neto,Cesar de; Pinho,João Renato Rebello; Malta,Fernanda de Mello; Capuani,Ligia; Campos,Aléia FaustinaABSTRACT OBJECTIVE To investigate the HCV cascade of care and to identify the factors associated with loss or absence to follow-up of patients identified as infected with hepatitis C through blood donation. METHODS Blood donors from 1994 to 2012, identified with positive anti- HCV by enzyme immunoassay and immunoblot tests were invited to participate in the study, through letters or phone calls. Patients who ag...
Characteristics of an outpatient chronic hepatitis B virus infection cohort
Assis,Danyenne Rejane de; Tenore,Simone de Barros; Pinho,João Renato Rebello; Lewi,David Salomão; Ferreira,Paulo Roberto AbrãoABSTRACT Objective: To characterize a chronic hepatitis B cohort based on initial and follow-up clinical evaluations. Methods: A retrospective and descriptive analysis of clinical and laboratory data from chronic HBsAg adult carriers, without HIV, unexposed to treatment, with at least two outpatient visits, between February 2006 and November 2012. Fisher´s exact test, χ², Wilcoxon, Spearman, multiple comparison...
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hos...
Sitnik,Roberta; Marra,Alexandre Rodrigues; Petroni,Roberta Cardoso; Ramos,Ozires Pereira Santos; Martino,Marinês Dalla Valle; Pasternak,JacyrObjective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture.Methods A total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests.Results More positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast t...
Personalized medicine and the clinical laboratory
Pinho,João Renato Rebello; Sitnik,Roberta; Mangueira,Cristóvão Luis PitangueiraPersonalized medicine is the use of biomarkers, most of them molecular markers, for detection of specific genetic traits to guide various approaches for preventing and treating different conditions. The identification of several genes related to heredity, oncology and infectious diseases lead to the detection of genetic polymorphisms that are involved not only in different clinical progression of these diseases...
Detecção de mutações no gene KIT em leucemia mieloide aguda
Machado,Luis Eduardo Silva; Pinho,João Renato Rebello; Sitnik,Roberta; Muto,Nair Hideko; Velloso,Elvira Deolinda Rodrigues PereiraOBJETIVO: Descrever a metodologia para detecção de mutações nos éxons 8 e 17 do gene KIT em pacientes portadores de leucemia mieloide aguda, para implementação desse teste no laboratório clínico do Hospital Israelita Albert Einstein. MÉTODOS: Extração do DNA genômico de 54 amostras de sangue periférico ou medula óssea de pacientes com leucemia mieloide aguda para amplificação, por reação em cadeia da polimerase...
Molecular and cytogenetic abnormalities in acute myeloid leukemia: review and c...
Velloso,Elvira Deolinda Rodrigues Pereira; Motta,Carlos Henrique Ares Silveira da; Furtado,Juliana Braga; Bacal,Nydia StrachmanObjective: To study the frequency of mutations that may lead to a good or bad prognosis, as well as their relation with the karyotype and immunophenotype in patients with acute myeloid leukemia. Methods: Thirty samples of patients with acute myeloid leukemia were studied, in which FLT3-ITD, FLT3-TKD and NPM1 mutations were investigated. All samples were submitted to immunophenotyping and 25 to karyotyping. Resu...