15 documents found, page 1 of 2
Pharmacological NF-κB inhibition decreases cisplatin chemoresistance in muscle-...
Gil da Costa, Rui; Levesque, C; Bianchi-Frias D; Chatterjee, P; Lam, HM; Santos, C; Coleman, IM; Ferreirinha, P; Vilanova, M; Pinto da Cunha, NMost patients with muscle-invasive bladder cancer (MIBC) are not cured with platinum chemotherapy. Up-regulation of nuclear factor kappa light-chain enhancer of activated B cells (NF-κB) is a major mechanism underlying chemoresistance, suggesting that its pharmacological inhibition may increase platinum efficacy. NF-κB signaling was investigated in two patient cohorts. The Cancer Genome Atlas (TCGA) was used to...
Mannosylated glycans impair normal T-cell development by reprogramming commitme...
Vicente, MM; Alves, I; Fernandes, Â; Dias, AM; Santos-Pereira, B; Pérez-Anton, E; Santos, S; Yang, T; Correia, A; Münster-Kühnel, A; Almeida, ARMT-cell development ensures the formation of diverse repertoires of T-cell receptors (TCRs) that recognize a variety of antigens. Glycosylation is a major posttranslational modification present in virtually all cells, including T-lymphocytes, that regulates activity/functions. Although these structures are known to be involved in TCR-selection in DP thymocytes, it is unclear how glycans regulate other thymic dev...
Dectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-G...
Pedro, ARV; Lima, T; Fróis-Martins, R; Leal, B; Ramos, IC; Martins, EG; Cabrita, ARJ; Fonseca, AJM; Maia, MRG; Vilanova, M; Correia, AYeast-derived products containing β-glucans have long been used as feed supplements in domesticated animals in an attempt to increase immunity. β-glucans are mainly recognized by the cell surface receptor CLEC7A, also designated Dectin-1. Although the immune mechanisms elicited through Dectin-1 activation have been studied in detail in mice and humans, they are poorly understood in other species. Here, we evalu...
Fighting Staphylococcus epidermidis Biofilm-Associated Infections: Can Iron Be ...
Oliveira, F; Rohde, H; Vilanova, M; Cerca, NStaphylococcus epidermidis is one of the most important commensal microorganisms of human skin and mucosae. However, this bacterial species is also the cause of severe infections in immunocompromised patients, specially associated with the utilization of indwelling medical devices, that often serve as a scaffold for biofilm formation. S. epidermidis strains are often multidrug resistant and its association with...
Vaccines in Congenital Toxoplasmosis: Advances and Perspectives
Barros, M; Teixeira, D; Vilanova, M; Correia, A; Teixeira, N; Borges, MCongenital toxoplasmosis has a high impact on human disease worldwide, inducing serious consequences from fetus to adulthood. Despite this, there are currently no human vaccines available to prevent this infection. Most vaccination studies against Toxoplasma gondii infection used animal models in which the infection was established by exogenous inoculation. Here, we review recent research on potential T. gondii...
Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formatio...
Oliveira, F; Lima, T; Correia, A; Silva, AM; Soares, C; Morais, S; Weißelberg, S; Vilanova, M; Rohde, H; Cerca, NIron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there is currently no comprehensive study addressing siderophore production in Staphylococcus epidermidis. Staphylococcus...
Effect of zinc source and exogenous enzymes supplementation on zinc status in d...
Pereira, AM; Guedes, M; Matos, E; Pinto, E; Almeida, AA; Segundo, MA; Correia, A; Vilanova, M; Fonseca, AJM; Cabrita, ARJZinc is an essential element, a cofactor of many enzymes, and performs catalytic, structural and regulatory functions. Once in the gastrointestinal tract, zinc can interact with food constituents. Phytic acid, the major phosphorus storage in plants, limits zinc availability from animal feeds due to the formation of insoluble complexes with phytates. This study tested the effect of supplemental zinc source (zinc...
Understanding the Lipid and Protein Corona Formation on Different Sized Polymer...
Lima, T; Bernfur, K; Vilanova, M; Cedervall, TWhen in contact with biological fluids, nanoparticles dynamically absorb biomolecules like proteins and lipids onto their surface, forming a “corona”. This biocorona is a dynamic and complex structure that determines how host cells respond to nanoparticles. Despite the common use of mouse models in pre-clinical and toxicological experiments, the impact of corona formed in mouse serum on the biophysical and biol...
A live auxotrophic vaccine confers mucosal immunity and protection against leth...
Cabral, MP; Correia, A; Vilanova, M; Gärtner, F; Moscoso, M; García, P; Vallejo, JA; Pérez, A; Francisco-Tomé, M; Fuentes-Valverde, V; Bou, GPseudomonas aeruginosa is one of the leading causes of nosocomial pneumonia and its associated mortality. Moreover, extensively drug-resistant high-risk clones are globally widespread, presenting a major challenge to the healthcare systems. Despite this, no vaccine is available against this high-concerning pathogen. Here we tested immunogenicity and protective efficacy of an experimental live vaccine against P....
Inhalation of Bacterial Cellulose Nanofibrils triggers an inflammatory response...
Silva-Carvalho, R; Silva, JP; Ferreirinha, P; Leitão, AF; Andrade, FK; Costa, RMG; Cristelo, C; Rosa, MF; Vilanova, M; Gama, FMIn view of the growing industrial use of Bacterial cellulose (BC), and taking into account that it might become airborne and be inhaled after industrial processing, assessing its potential pulmonary toxic effects assumes high relevance. In this work, the murine model was used to assess the effects of exposure to respirable BC nanofibrils (nBC), obtained by disintegration of BC produced by Komagataeibacter hanse...