92 documents found, page 1 of 10
Deletion of codY impairs Staphylococcus epidermidis biofilm formation, generati...
Lopes, Nathalie; Pereira, Renato B; Correia, Alexandra; Vilanova, Manuel; Cerca, Nuno; França, Ângela Maria Oliveira SousaIntroduction. Staphylococcus epidermidis biofilms are one of the major causes of bloodstream infections related to the use of medical devices. The diagnosis of these infections is challenging, delaying their treatment and resulting in increased morbidity and mortality rates. As such, it is urgent to characterize the mechanisms employed by this bacterium to endure antibiotic treatments and the response of the ho...
Design of a lipid nano-delivery system containing recombinant Candida albicans ...
Costa-Barbosa, Augusto; Pacheco, Maria Inês; Carneiro, Catarina; Botelho, Cláudia M.; Gomes, Andreia C; Real Oliveira, M. Elisabete C.D.; Collins, TonyOpportunistic fungi cause lethal systemic infections and impose high medical costs to health systems. The World Health Organization has recognized the importance of fungal infections, including them in its global priority list guiding research, development, and discovery of new therapeutic approaches. Fungal vaccine development has been proposed as one of the treatment and prevention strategies in the last deca...
Involvement of the Iron-Regulated Loci hts and fhuC in Biofilm Formation and Su...
Oliveira, Fernando; Lima, Tânia; Correia, Alexandra; Silva, Ana Margarida; Soares, Cristina; Morais, Simone; Weißelberg, Samira; Vilanova, ManuelStaphylococcus epidermidis is a major nosocomial pathogen with a remarkable ability to persist on indwelling medical devices through biofilm formation. Nevertheless, it remains intriguing how this process is efficiently achieved under the host’s harsh conditions, where the availability of nutrients, such as essential metals, is scarce. Following our previous identification of two iron-regulated loci putatively ...
mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence p...
Gaio, Vânia Silva; Lima, Tânia; Vilanova, Manuel; Cerca, Nuno; França, Ângela Maria Oliveira SousaStaphylococcus epidermidis biofilm cells are characterized by increased antimicrobial tolerance and improved ability to evade host immune system defenses. These features are, in part, due to the presence of viable but non-culturable (VBNC) cells. A previous study identified genes potentially involved in VBNC cells formation in S. epidermidis biofilms, among which SERP1682/1681 raised special interest due to the...
Involvement of the iron-regulated Loci hts and fhuCin biofilm formation and sur...
Oliveira, Fernando Eduardo Freitas; Lima, Tânia; Correia, Alexandra; Silva, Ana Margarida; Soares, Cristina; Morais, Simone; Weißelberg, SamiraStaphylococcus epidermidis is one of the most important nosocomial pathogens and a major cause of central line-associated bloodstream infections. Once in the bloodstream, this bacterium must surpass severe iron restriction in order to survive and establish infection. Staphylococcus epidermidis is a major nosocomial pathogen with a remarkable ability to persist on indwelling medical devices through biofilm forma...
Isolation and identification of an arabinogalactan extracted from pistachio ext...
Hamed, Mariem; Coelho, Elisabete; Bastos, Rita; Evtuguin, Dmitry V; Ferreira, Sónia S; Lima, Tânia; Vilanova, Manuel; Sila, Assaad; Coimbra, Manuel AThis work studies the extraction and purification of a novel arabinogalactan from pistachio external hull. It was extracted with a simple method from pistachio hull which is considered as unexploited waste. Based on the results of sugar analysis by GC-FID, glycosidic linkage by GC-MS, NMR spectroscopy, and molecular weight by Size Exclusion Chromatography, pistachio hull water soluble polysaccharides (PHWSP) we...
Fighting Staphylococcus epidermidis biofilm-associated infections: can iron be ...
Oliveira, Fernando Eduardo Freitas; Rohde, Holger; Vilanova, Manuel; Cerca, NunoStaphylococcus epidermidis is one of the most important commensal microorganisms of human skin and mucosae. However, this bacterial species is also the cause of severe infections in immunocompromised patients, specially associated with the utilization of indwelling medical devices, that often serve as a scaffold for biofilm formation. S. epidermidis strains are often multidrug resistant and its association with...
Siderophore-mediated iron acquisition plays a critical role in biofilm formatio...
Oliveira, Fernando Eduardo Freitas; Lima, Tânia; Correia, Alexandra; Silva, Ana Margarida; Soares, Cristina; Morais, Simone; Weißelberg, SamiraIron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there is currently no comprehensive study addressing siderophore production in Staphylococcus epidermidis. Staphylococcus...
The emerging role of iron acquisition in biofilm-associated infections
Oliveira, Fernando E.; Rohde, Holger; Vilanova, Manuel; Cerca, NunoA possible association between iron and biofilm formation has been explored for a long time. Here, we focus on major recent advances that shed light on the mechanisms behind this relationship and discuss how siderophore-mediated iron acquisition may impact the virulence of important nosocomial pathogens.
Inflammatory cell recruitment in Candida glabrata biofilm cell-infected mice re...
Rodrigues, Célia F.; Correia, Alexandra; Vilanova, Manuel; Henriques, Mariana(1) Background: Due to a high rate of antifungal resistance, Candida glabrata is one of the most prevalent Candida spp. linked to systemic candidiasis, which is particularly critical in catheterized patients. The goal of this work was to simulate a systemic infection exclusively derived from C. glabrata biofilm cells and to evaluate the effectiveness of the treatment of two echinocandinscaspofungin (Csf) and mi...