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Generation of a library of carbohydrate-active enzymes for plant biomass decons...
Cardoso, Vânia; Bras, Joana L. A.; Costa, Ines F.; Ferreira, Luis M. A.; Gama, Luis; Vincentelli, Renaud; Henrissat, Bernard; Fontes, Carlos M.G.A.In nature, the deconstruction of plant carbohydrates is carried out by carbohydrate-active enzymes (CAZymes). A high-throughput (HTP) strategy was used to isolate and clone 1476 genes obtained from a diverse library of recombinant CAZymes covering a variety of sequence-based families, enzyme classes, and source organisms. All genes were successfully isolated by either PCR (61%) or gene synthesis (GS) (39%) and ...
Date: 2022
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Origin: Repositório da Universidade de Lisboa