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Utilização do eluato de sangue dessecado em papel de filtro no diagnóstico soro...

Foi realizada pesquisa de anticorpos anti-Toxoplasmagondii em 183 amostras de sangue dessecado em papel de filtro utilizando as reações de imunofluorescência indireta ELISA e dot-ELIS A, tomando como referência os resultados obtidos nos soros. A análise dos resultados demonstrou que papéis com sangue dessecado podem ser conservados por um período mínimo de 45 dias à temperatura ambiente e por seis meses a 4°C, ...

Diagnosis of human toxoplasmosis by a dot enzyme-linked immunosorbent assay.

Validity of serology for american trypanosomiasis with eluates from filter paper.

Relationship of Leishmania-specific IgG levels and IgG avidity with parasite de...

The clinical status and tissue parasite burden of the skin and spleen of 40 dogs naturally infected with Leishmania chagasi (syn. Leishmania infantum), together with 5 uninfected control dogs, were assessed. On the basis of the clinical evaluation, infected dogs were classified as asymptomatic (AD) or symptomatic (SD). Infected animals were also grouped according to their parasite load as exhibiting low (LP), m...

Susceptibility to re-infection in C57BL/6 mice with recombinant strains of Toxo...

This work reports results of re-infection of BALB/c and C57BL/6 mice with different recombinant strains of Toxoplasma gondii. Mice were prime-infected with the non-virulent D8 strain and challenged with virulent strains. PCR–RFLP of cS10-A6 genetic marker of T. gondii demonstrated that BALB/c mice were reinfected with the EGS strain, while C57BL/6 mice were re-infected with the EGS and CH3 strains. Levels of IF...

Utilização do eluato de sangue dessecado em papel de filtro no diagnóstico soro...

Eluates of 183 dried caprine blood samples on filter paper were tested for anti-Toxoplasma gondii antibodies, using IFA, ELISA and dot-ELISA, and compared with results obtained by direct observations on comparable sera, usin the same tests. The results has showed that papers with dried blood can be preserved for up to 45 days at room temperature and for six months at 4°C, provided they are protected against hum...

Diagnóstico da toxoplasmose humana através de um tese imunoenzimático Dot-ELISA

O Dot-ELISA foi padronizado e avaliado para o imunodiagnóstico da toxoplasmose humana. Entre 538 amostras testadas pela reação de Imunofluorescência Indireta (teste de referência) 183 foram positivas considerando-se os soros reagentes nas diluições >; 1:16 (34%). Utilizando a diluição 1:256 como título discriminante para o Dot-ELISA, foram observados 192 soros positivos (36%). A comparação entre as duas técnica...