82 documents found, page 1 of 9
Influenza A virus propagation requires the activation of the unfolded protein r...
Marques, Mariana; Ramos, Bruno; Albuquerque, Hélio; Pereira, Marisa; Ribeiro, Diana Roberta; Nunes, Alexandre; Sarabando, Jéssica; Brás, DanielaInfluenza A virus (IAV) employs multiple strategies to manipulate cellular mechanisms and support proper virion formation and propagation. In this study, we performed a detailed analysis of the interplay between IAV and the host cells’ proteostasis throughout the entire infectious cycle. We reveal that IAV infection activates the inositol requiring enzyme 1 (IRE1) branch of the unfolded protein response, and th...
Integrating Multi-Omics Analysis for Enhanced Diagnosis and Treatment of Gliobl...
Barzegar Behrooz, Amir; Latifi-Navid, Hamid; Silva Rosa, Simone C. da; Swiat, Maciej; Wiechec, Emilia; Vitorino, Carla; Vitorino, Rui; Jamalpoor, ZahraThe most aggressive primary malignant brain tumor in adults is glioblastoma (GBM), which has poor overall survival (OS). There is a high relapse rate among patients with GBM despite maximally safe surgery, radiation therapy, temozolomide (TMZ), and aggressive treatment. Hence, there is an urgent and unmet clinical need for new approaches to managing GBM. The current study identified modules (MYC, EGFR, PIK3CA, ...
A two-pronged approach against glioblastoma: drug repurposing and nanoformulati...
Mendes, Maria; Branco, Francisco; Vitorino, Rui; Sousa, João; Pais, Alberto; Vitorino, CarlaGlioblastoma (GB) is one of the most lethal types of neoplasms. Its biologically aggressive nature and the presence of the blood-brain barrier (BBB) limit the efficacy of standard therapies. Several strategies are currently being developed to both overcome the BBB and deliver drugs site specifically to tumor cells. This work hypothesizes a two-pronged approach to tackle GB: drug repurposing with celecoxib (CXB)...
Expression of NORAD correlates with breast cancer aggressiveness and protects b...
Alves-Vale, Catarina; Capela, Ana Maria; Tavares-Marcos, Carlota; Domingues-Silva, Beatriz; Pereira, Bruno; Santos, Francisco; Gomes, Carla PereiraThe recently discovered human lncRNA NORAD is induced after DNA damage in a p53-dependent manner. It plays a critical role in the maintenance of genomic stability through interaction with Pumilio proteins, limiting the repression of their target mRNAs. Therefore, NORAD inactivation causes chromosomal instability and aneuploidy, which contributes to the accumulation of genetic abnormalities and tumorigenesis. NO...
A peptide-centric approach to analyse quantitative proteomics data- an applicat...
Lima, Tania; Rodrigues, João Eduardo; Manadas, Bruno; Henrique, Rui; Fardilha, Margarida; Vitorino, RuiBottom-up proteomics is a popular approach in molecular biomarker research. However, protein analysts have realized the limitations of protein-based approaches for identifying and quantifying proteins in complex samples, such as the identification of peptides sequences shared by multiple proteins and the difficulty in identifying modified peptides. Thus, there are many exciting opportunities to improve analysis...
Galectin-3 in prostate cancer and heart diseases: a biomarker for these two fri...
Lima, Tânia; Perpétuo, Luís; Henrique, Rui; Fardilha, Margarida; Leite-Moreira, Adelino; Bastos, Jose; Vitorino, RuiGalectin-3 (Gal-3) belongs to galectin protein family, a type of β-galactose-binding lectin having more than one evolutionarily conserved domain of carbohydrate recognition. Gal-3 is mainly located in the cytoplasm, but it also enters the nucleus and is secreted into the extracellular environment and biological fluids such as urine, saliva, and serum. It plays an important role in many biological functions, suc...
Gal-3 Protein Expression and Localization in Prostate Tumours
Lima, Tânia; Macedo-Silva, Catarina; Felizardo, Diana; Fraga, João; Carneiro, Isa; Jerónimo, Carmen; Henrique, Rui; Fardilha, Margarida; Vitorino, RuiGal-3 plays an important role in cell survival, mRNA splicing, and cell-cell and cell-matrix interactions. Depending on its cellular localization and cancer type, Gal-3 may have tumour-suppressive or tumour-promoting activities. Given the promising diagnostic role of Gal-3 in the urine of PCa patients found in our previous study, its concordant gene and protein expression levels, and its involvement in PCa-rela...
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a sim...
António, Maria; Lima, Tânia; Ferreira, Rita; Fardilha, Margarida; Bastos, José Mesquita; Vitorino, Rui; Daniel-da-Silva, Ana L.Galectin-3 (Gal-3) is a carbohydrate-binding protein associated with the development and progress of heart failure. Here, we report the first colorimetric and low-cost approach for detecting and quantifying Gal-3 using gold nanoparticles (AuNPs) bioconjugated with Gal-3 antibody. The interaction of Gal-3 with the resulting nanoprobes led to a linear response of the absorbance ratio A750nm/A526nm to Gal-3 concen...
Diagnostic and monitoring applications using Near infrared (NIR) Spectroscopy i...
Vitorino, Rui; Barros, António S.; Guedes, Sofia; Caixeta, Douglas C.; Sabino-Silva, RobinsonEarly cancer diagnosis plays a critical role in improving treatment outcomes and increasing survival rates for certain cancers. NIR spectroscopy offers a rapid and cost-effective approach to evaluate the optical properties of tissues at the microvessel level and provides valuable molecular insights. The integration of NIR spectroscopy with advanced data-driven algorithms in portable instruments has made it a cu...
Exercise training decreases the load and changes the content of circulating SDS...
Gouveia, Marisol; Schmidt, Cristine; Basilio, Priscilla Gois; Aveiro, Susana; Domingues, Pedro; Xia, Ke; Colón, Wilfredo; Vitorino, Rui; Ferreira, RitaBackgroundHeart failure (HF) often disrupts the protein quality control (PQC) system leading to protein aggregate accumulation. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis.AimTo determine the effects of exercise training on the load and composition of plasma SDS-resistant protein aggregates (SRA) in patients with...