10 documents found, page 1 of 1

The neglected contribution of streptomycin to the tuberculosis drug resistance ...

The airborne pathogen Mycobacterium tuberculosis is responsible for a present major public health problem worsened by the emergence of drug resistance. M. tuberculosis has acquired and developed streptomycin (STR) resistance mechanisms that have been maintained and transmitted in the population over the last decades. Indeed, STR resistant mutations are frequently identified across the main M. tuberculosis linea...

Emergence of Multidrug-Resistant Mycobacterium Tuberculosis of the Beijing Line...

The Beijing genotype comprises a highly disseminated strain type that is frequently associated with multidrug resistant (MDR) tuberculosis (TB) and increased transmissibility but, countries such as Portugal and Guinea-Bissau fall outside the regions phylogeographically associated with this specific genotype. Nevertheless, recent data shows that this genotype might be gradually emerging in these two countries as...

Using Genomics to Understand the Origin and Dispersion of Multidrug and Extensi...

Portugal is a low incidence country for tuberculosis (TB) disease. Now figuring among TB low incidence countries, it has since the 1990s reported multidrug resistant and extensively drug resistant (XDR) TB cases, driven predominantly by two strain-types: Lisboa3 and Q1. This study describes the largest characterization of the evolutionary trajectory of M/XDR-TB strains in Portugal, spanning a time-period of two...

Experimental Evidence for Limited in vivo Virulence of Mycobacterium africanum

Tuberculosis remains a public health problem and a main cause of death to humans. Both Mycobacterium tuberculosis and Mycobacterium africanum cause tuberculosis. In contrast to M. tuberculosis, which is geographically spread, M. africanum is restricted to West Africa. Differences have also been found in the growth rate and type of disease caused by M. africanum, globally suggesting an attenuation of this bacter...

Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium...

Background: Approximately 10% of the Mycobacterium tuberculosis genome is made up of two families of genes that are poorly characterized due to their high GC content and highly repetitive nature. The PE and PPE families are typified by their highly conserved N-terminal domains that incorporate proline-glutamate (PE) and proline-proline-glutamate (PPE) signature motifs. They are hypothesised to be important viru...

Isolation of terpenic compounds from the methanolic extract of Euphorbia lagasc...

For as long as man can remember, plants have been used worldwide for the treatment of diseases. Today, many of the drugs currently used are derived from natural products or have dependent upon a natural product for their development and the recent discoveries of the antimalarial artemisinin and the anticancer agent taxol indicate the continuing importance of plant species in drug discovery.2 In recent years, a ...

New efflux pumps inhibitors for Gram positive bacteria strains and cancer cells...

All living cells contain genes encoding multidrug transporters and some of them play an important role in conferring drug resistance in mammalian cancer cells and in microbial pathogens such as Staphylococcus aureus, Enterococcus faecalis, Candida albicans, Plasmodium falciparum, and Leishmania donovani. The over-expression of P- glycoprotein (P-gp) is one of the principal mechanisms of multidrug resistance (MD...

Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identific...

The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensit...

Isolation of terpenic compounds from the methanolic extract of Euphorbia lagasc...

Isoniazid-induced transient high-level resistance in Mycobacterium tuberculosis

An American Type Culture Collection reference strain and eight clinical strains of Mycobacterium tuberculosis, all of which were susceptible to isoniazid (INH) (mean MIC, 0.06 mg/liter) and negative for the Ser315Thr katG mutation, were left in their BACTEC 12B vials (for use with the BACTEC 460-TB method) containing 0.1 mg of INH per liter for periods of up to 28 days after the completion of the antibiotic sus...