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Monitoring Leishmania infection and exposure to Phlebotomus perniciosus using m...

Maia, Carla; Cristóvão, José; Pereira, André; Kostalova, Tatiana; Lestinova, Tereza; Sumova, Petra; Volf, Petr; Campino, Lenea

BACKGROUND: In endemic areas of zoonotic leishmaniosis caused by L. infantum, early detection of Leishmania infection in dogs is essential to control the dissemination of the parasite to humans. The aim of this study was to evaluate the serological and/or molecular diagnostic performance of minimally and non-invasive samples (conjunctiva cells (CS) and peripheral blood (PB)) for monitoring Leishmania infection/...


Antibody response to Phlebotomus perniciosus saliva in cats naturally exposed t...

Pereira, André; Cristóvão, José Manuel; Vilhena, Hugo; Martins, Ângela; Cachola, Patrícia; Henriques, Joaquim; Coimbra, Mónica; Catarino, Ana

BACKGROUND: Zoonotic leishmaniosis, caused by the protozoan Leishmania infantum, is a public and animal health problem in Asia, Central and South America, the Middle East and the Mediterranean Basin. Several phlebotomine sand fly species from the subgenus Larroussius are vectors of L. infantum. Data from dogs living in endemic areas of leishmaniosis advocate the use of antibody response to phlebotomine sand fly...


Seasonal Dynamics of Phlebotomine Sand Fly Species Proven Vectors of Mediterran...

Alten, Bulent; Maia, Carla; Afonso, Maria Odete; Campino, Lenea; Jiménez, Maribel; González, Estela; Molina, Ricardo; Bañuls, Anne Laure

BACKGROUND: The recent geographical expansion of phlebotomine vectors of Leishmania infantum in the Mediterranean subregion has been attributed to ongoing climate changes. At these latitudes, the activity of sand flies is typically seasonal; because seasonal phenomena are also sensitive to general variations in climate, current phenological data sets can provide a baseline for continuing investigations on sand ...


Exposure to Leishmania spp. and sand flies in domestic animals in northwestern ...

Rohousova, Iva; Talmi-Frank, Dalit; Kostalova, Tatiana; Polanska, Nikola; Lestinova, Tereza; Kassahun, Aysheshm; Yasur-Landau, Daniel; Maia, Carla

WOS:000357470300003 PMID: 26152578; Background: Human visceral leishmaniasis caused by Leishmania donovani is considered an anthroponosis; however, Leishmania-infected animals have been increasingly reported in L. donovani foci, and the role of these animals as reservoirs for human L. donovani infection remains unclear. Methods: We conducted a study of domestic animals (goats, sheep, cows, dogs, and donkeys) in...


Experimental transmission of leishmania infantum by two major vectors

Maia, Carla; Seblova, Veronika; Sadlova, Jovana; Votypka, Jan; Volf, Petr

We quantified Leishmania infantum parasites transmitted by natural vectors for the first time. Both L. infantum strains studied, dermotropic CUK3 and viscerotropic IMT373, developed well in Phlebotomus perniciosus and Lutzomyia longipalpis. They produced heavy late-stage infection and colonized the stomodeal valve, which is a prerequisite for successful transmission. Infected sand fly females, and especially th...


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