2 documents found, page 1 of 1
Comparison of polymerase chain reaction with other laboratory methods for the d...
Marques, Marcos José; Volpini, Ângela Cristina; Coelho, George Luiz Lins Machado; Pinto, Jackson Machado; Costa, Carlos Alberto da; Mayrink, WilsonAn evaluation of 5 laboratory methods for diagnosing American cutaneous leishmaniasis (ACL) was carried out on patients from an endemic area of Brazil. From 164 patients presenting cutaneous lesions, and suspected to have ACL, 133 (81.1%) were confirmed for the disease by Montenegro skin test (MST) and/or parasitologic examination (PE). In both groups of patients, the positivity of polymerase chain reaction (PC...
Use of PCR–RFLP to identify Leishmania species in naturally-infected dogs.
Andrade, Hélida Monteiro de; Reis, Alexandre Barbosa; Santos, Sara Lopes dos; Volpini, Ângela Cristina; Marques, Marcos José; Romanha, Alvaro JoséTissue imprints on Giemsa stained slides from dogs were used to investigate the presence of Leishmania amastigotes by either optical microscopy (OM) or Polymerase chain reaction (PCR) detection of DNA. Samples from skin, spleen, lymph node, liver and bone marrow from a Leishmaniasis endemic area dogs where Leishmania ( Leishmania ) chagasi and Leishmania ( Viannia) braziliensis are sympatric were studied. Dogs ...