5 documents found, page 1 of 1
Identification, characterization, and antifungal activity of cysteine peptidase...
Freitas, Cleverson D. T.; Silva, Rafaela O; Ramos, Márcio Viana; Porfírio, Camila T. M. N.; Farias, Davi F.; Sousa, Jeanlex S; Oliveira, João P. B.Cysteine peptidases (EC 3.4.22) are the most abundant enzymes in latex fluids. However, their physiological functions are still poorly understood, mainly related to defense against phytopathogens. The present study reports the cDNA cloning and sequencing of five undescribed cysteine peptidases from Calotropis procera (Aiton) Dryand (Apocynaceae) as well as some in silico analyses. Of these, three cysteine pepti...
Biotechnological potential of a cysteine protease (CpCP3) from Calotropis proce...
Silva, Maria Z. R.; Oliveira, João P. B.; Ramos, Márcio Viana; Farias, Davi F.; Sá, Chayenne A. de; Ribeiro, Juliana A. C.; Silva, Ayrles F. B.This article reports the characterization and evaluation of the biotechnological potential of a cysteine protease purified from Calotropis procera (CpCP3). This enzyme was highly stable to different metal ions and was able to hydrolyze κ-casein similarly to bovine chymosin. Atomic force microscopy showed that the process of casein micelle aggregation induced by CpCP3 was similar to that caused by chymosin. The ...
Food safety assessment of an antifungal protein from Moringa oleifera seeds in ...
Pinto, Clidia E. M.; Farias, Davi F.; Carvalho, Ana F. U; Oliveira, Jose T. A.; Pereira, Mirella L.; Grangeiro, Thalles B.; Freire, Jose E. C.Mo-CBP3 is an antifungal protein produced by Moringa oleifera which has been investigated as potential candidate for developing transgenic crops. Before the use of novel proteins, food safety tests must be conducted. This work represents an early food safety assessment of Mo-CBP3, using the two-tiered approach proposed by ILSI. The history of safe use, mode of action and results for amino acid sequence homology...
A protein isolate from Moringa oleifera leaves has hypoglycemic and antioxidant...
Paula, Paulo C.; Sousa, Daniele de Oliveira Bezerra de; Oliveira, José Tadeu Abreu de; Carvalho, Ana Fontenele Urano; Alves, Bella Giselly TorresMoringa oleifera has been used in traditional medicine to treat diabetes. However, few studies have been conducted to relate its antidiabetic properties to proteins. In this study, a leaf protein isolate was obtained from M. oleifera leaves, named Mo-LPI, and the hypoglycemic and antioxidant effects on alloxan-induced diabetic mice were assessed. Mo-LPI was obtained by aqueous extraction, ammonium sulphate prec...
Larvicidal activity of the water extract of Moringa oleifera seeds against Aede...
Ferreira, Paulo M. P.; Carvalho, Ana F. U.; Farias, Davi F.; Cariolano, Nara G.; Melo, Vânia M. M.; Queiroz, Maria G. R.; Martins, Alice M. C.Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:49:34Z No. of bitstreams: 1 S0001-37652009000200007.pdf: 536011 bytes, checksum: 846642c2588a4bd24f90ee703c7ddc86 (MD5); Made available in DSpace on 2013-08-22T18:49:35Z (GMT). No. of bitstreams: 1 S0001-37652009000200007.pdf: 536011 bytes, checksum: 846642c2588a4bd24f90ee703c7ddc86 (MD5) Previous issue date: 2009-06-01; Made availabl...