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Collagenolytic enzymes produced by fungi: a systematic review

Wanderley, M.; Neto, José Manoel Werley Duarte; Filho, José Luiz de Lima; Lima, Carolina de Albuquerque; Teixeira, J. A.; Porto, Ana Lúcia Figueiredo

Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenas...


Purification and characterization of a collagenase from Penicillium sp. UCP 128...

Wanderley, M.; Neto, José Manoel Wanderley Duarte; Albuquerque, Wendell Wagner Campos; Marques, Daniela de Araújo Viana; Lima, Carolina de Albuquerque

Collagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional methods including...


Production and characterization of collagenase by Penicillium sp. UCP 1286 isol...

Wanderley, M.; Neto, José Manoel Wanderley Duarte; Lima, Carolina de Albuquerque; Silvério, Sara Isabel Cruz; Filho, José Luiz de Lima; Teixeira, J. A.

A new Penicillium sp. strain isolated from the soil of Caatinga, a Brazilian Biome (UCP 1286) was selected for collagenase production. Fermentation system allowing obtention of collagenolytic activity about 2.7 times higher than existing data, with the highest values of collagenolytic and specific activity (379.80 U/mL, 1460.77 U/mg, respectively), after 126 hours. Applying a factorial design, enzyme production...


Towards a multimodal analysis of European piano schools of music performance

Lourenço, Sofia; Martins, L. G.; Tits, M.; Wanderley, M.; Megre, R.

This study aims to characterize representative performances by experienced pianists in order to determine main influential trends in performance, derived specifically from traditional piano practices referred to as National Piano Schools. The methodology of this exploratory study departs from a musicological empirical analysis in articulation with recent technological developments for metric methods. It allowed...


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