10 documents found, page 1 of 1
Cytokine-Based Insights into Bloodstream Infections and Bacterial Gram Typing i...
Araújo, Rúben; Ramalhete, Luís; Von Rekowski, Cristiana; Fonseca, Tiago AH; Calado, Cecília; Bento, LuísFunding Information: This research was funded by project grant DSAIPA/DS/0117/2020, supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia, Portugal, and IPL/IDI&CA2024/R-DICIP_ISEL by Instituto Polit\u00E9cnico de Lisboa, Portugal. The present work was conducted in Instituto Polit\u00E9cnico de Lisboa and in the Engineering & Health Laboratory, resulting from a collaboration protocol established be...
Simplifying data analysis in biomedical research: an automated, user-friendly tool
Araújo, Rúben; Ramalhete, Luís; Viegas, Ana; Von Rekowski, Cristiana; Fonseca, Tiago AH; Calado, Cecília; Bento, LuísRobust data normalization and analysis are pivotal in biomedical research to ensure that observed differences in populations are directly attributable to the target variable, rather than dispari ties between control and study groups. ArsHive addresses this challenge using advanced algorithms to normalize populations (e.g., control and study groups) and perform statistical evaluations between demographic, clinic...
Early mortality prediction in intensive care unit patients based on serum metab...
Araújo, Rúben; Ramalhete, Luís; Von Rekowski, Cristiana; Fonseca, Tiago AH; Bento, Luís; Calado, CecíliaPredicting mortality in intensive care units (ICUs) is essential for timely interventions and efficient resource use, especially during pandemics like COVID-19, where high mortality persisted even after the state of emergency ended. Current mortality prediction methods remain limited, especially for critically ill ICU patients, due to their dynamic metabolic changes and heterogeneous pathophysiological processe...
A new method for a rapid and high-throughput comparison of molecular profiles a...
Caldeira, Viviana; Fonseca, Tiago AH; N'Dembo, Luana; Araújo, Rúben; Von Rekowski, Cristiana; Sampaio, Pedro; Calado, CecíliaTo robustly discover and explore phytocompounds, it is necessary to evaluate the interrelationships between the plant species, plant tissue, and the extraction process on the extract composition and to predict its cytotoxicity. The present work evaluated how Fourier Transform InfraRed spectroscopy can acquire the molecular profile of aqueous and ethanol-based extracts obtained from leaves, seeds, and flowers of...
The characteristics and laboratory findings of SARS-CoV-2 infected patients dur...
Von Rekowski, Cristiana; Fonseca, Tiago AH; Araújo, Rúben; Brás-Geraldes, Carlos; Calado, Cecília; Bento, Luís; Pinto, IolaBackground and Objectives: Given the wide spectrum of clinical and laboratory manifestations of the coronavirus disease 2019 (COVID-19), it is imperative to identify potential contributing factors to patients’ outcomes. However, a limited number of studies have assessed how the different waves affected the progression of the disease, more so in Portugal. Therefore, our main purpose was to study the clinical and...
Discovery of delirium biomarkers through minimally invasive serum molecular fin...
Viegas, Ana; Araújo, Rúben; Ramalhete, Luís; Von Rekowski, Cristiana; Fonseca, Tiago AH; Bento, Luís; Calado, CecíliaDelirium presents a significant clinical challenge, primarily due to its profound impact on patient outcomes and the limitations of the current diagnostic methods, which are largely subjective. During the COVID-19 pandemic, this challenge was intensified as the frequency of delirium assessments decreased in Intensive Care Units (ICUs), even as the prevalence of delirium among critically ill patients increased. ...
The impact of the serum extraction protocol on metabolomic profiling using UPLC...
Fonseca, Tiago AH; Von Rekowski, Cristiana; Araújo, Rúben; Oliveira, Maria Da Conceiçao; Justino, Gonçalo C.; Bento, Luís; Calado, CecíliaBiofluid metabolomics is a very appealing tool to increase the knowledge associated with pathophysiological mechanisms leading to better and new therapies and biomarkers for disease diagnosis and prognosis. However, due to the complex process of metabolome analysis, including the metabolome isolation method and the platform used to analyze it, there are diverse factors that affect metabolomics output. In the pr...
Alternative sérum biomarkers of bacteraemia for intensive care unit patients
Araújo, Rúben; Von Rekowski, Cristiana; Bento, Luís; Fonseca, Tiago AH; Calado, CecíliaThe diagnosis of infections in hospital or clinical settings usually involves a series of time-consuming steps, including biological sample collection, culture growth of the organism isolation and subsequent characterization. For this, there are diverse infection biomarkers based on blood analysis, however, these are of limited use in patients presenting confound processes as inflammatory process as occurring a...
Comparison of analytical methods of serum untargeted metabolomics
Fonseca, Tiago AH; Araújo, Rúben; Von Rekowski, Cristiana; Justino, Gonçalo C.; Oliveira, Maria Da Conceiçao; Bento, Luís; Calado, CecíliaMetabolomics has emerged as a powerful tool in the discovery of new biomarkers for medical diagnosis and prognosis. However, there are numerous challenges, such as the methods used to characterize the system metabolome. In the present work, the comparison of two analytical platforms to acquire the serum metabolome of critically ill patients was conducted. The untargeted serum metabolome analysis by ultraperform...
Infection biomarkers based on metabolomics
Araújo, Rúben; Bento, Luís; Fonseca, Tiago AH; Von Rekowski, Cristiana; Ribeiro Da Cunha, Bernardo; Calado, CecíliaCurrent infection biomarkers are highly limited since they have low capability to predict infection in the presence of confounding processes such as in non-infectious inflammatory processes, low capability to predict disease outcomes and have limited applications to guide and evaluate therapeutic regimes. Therefore, it is critical to discover and develop new and effective clinical infection biomarkers, especial...