2 documents found, page 1 of 1
Combining nucleic acid mimics and spectral imaging with fluorescence in situ hy...
Azevedo, Andreia S.; Teixeira, H.; Ferreira, R.; Figueiredo, C.; Wengel, J.; Almeida, Carina; Azevedo, N. F.[Excerpt] Nucleic acid mimics (NAMs)- based assays, such as locked nucleic acid/2′-O- methyl-RNA- fluorescence in vivo hybridization (LNA/2′OMe- FISH), have been developed for the identification and spatial location of Helicobacter pylori directly in the stomach. While H. pylori is considered the main gastric pathogen, there is a diverse range of stomach colonizers that may be associated with disease in the sto...
Detection and discrimination of microorganisms using Locked Nucleic Acid - Fluo...
Azevedo, A. S.; Pereira, B.; Almeida, Carina; Melo, Luís F.; Wengel, J.; Azevedo, N. F.The impact of multispecies biofilms on catheter-associated urinary tract infections outcome is still unclear due to the lack of adequate methodologies to discriminate the populations in situ. Employing fluorescence in situ hybridization (FISH) to discriminate the populations in a biofilm, can contribute to the understanding of microorganisms interactions in this structures. Consequently, this information might ...