5 documents found, page 1 of 1
One-step purification of L-asparaginase from cell extracts using carbon xerogels
Raquel O. Cristóvão; R.A.M. Barros; Teresa P. Marramaque; Gonçalo G. Aguiar; Mafalda R. Almeida; Sónia A.C. Carabineiro; Gabriela B. de PaivaL-asparaginase (ASNase, EC 3.5.1.1) is an enzyme with wide applications in the pharmaceutical sector and food processing industries. It is mainly used as a biotherapeutic for treating Acute Lymphoblastic Leukemia (ALL) and to reduce acrylamide formation in starchy compounds. Despite its relevance, current purification methods for microbial enzymes involve complex and expensive techniques. To overcome this drawb...
Carbon-based nanomaterials for the development of anti-leukemic drugs
Rita Barros; Sónia A.C. Carabineiro; Mara G. Freire; Joaquim Luís Faria; Valéria C. Santos-Ebinuma; Ana P. M. Tavares; Claudia G Silva
New strategies for the removal of mycophenolate mofetil from aqueous solutions
Francisco, Rafael; Santos, Ana C. A.; Silva, Francisca A. e; Neves, Márcia C.; Sousa, Ana C.A.; Mara G. FreireMycophenolate Mofetil (MMF) is cytostatic drug acting as an inhibitor of inosine monophosphate dehydrogenase (IMPDH, classified by ATC/DDD as an immunosuppressant (L04AA06) that is used in the treatment of cancer and also to prevent organ rejection after hepatic, renal, and cardiac transplants. However, as with other cytostatic drugs, MMF is highly toxic and thus its handling and disposal is a matter of great c...
Development and characterization of a novell-asparaginase/MWCNT nanobioconjugate
Raquel O. Cristóvão; Mafalda R. Almeida; Maria A. Barros; João C. F. Nunes; Rui A. R. Boaventura; José M. Loureiro; Joaquim L. Faria; Márcia C. NevesThe enzymel-asparaginase (ASNase) presents effective antineoplastic properties used for acute lymphoblastic leukemia treatment besides their potential use in the food sector to decrease the acrylamide formation. Considering their applications, the improvement of this enzyme's properties by efficient immobilization techniques is in high demand. Carbon nanotubes are promising enzyme immobilization supports, since...
1H NMR and Molecular Dynamics Evidence for an Unexpected Interaction on the Ori...
Mara G. Freire; Catarina M. S. S. Neves; Artur M. S. Silva; Luís M. N. B. F. Santos; Isabel M. Marrucho; Luís P. N. Rebelo; Jindal K. ShahBy employing 1H NMR spectroscopy and molecular simulations, we provide an explanation for recent observations that the aqueous solubilities of ionic liquids exhibit salting-out to salting-in regimes upon addition of distinct inorganic salt ions. Using a typical ionic liquid [1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide], we observed the existence of preferential specific interactions between t...