10 documents found, page 1 of 1

The yeast protein kinase Sch9 functions as a central nutrient-responsive hub th...

Yeast cells are equipped with different nutrient signaling pathways that enable them to sense the availability of various nutrients and adjust metabolism and growth accordingly. These pathways are part of an intricate network since most of them are cross-regulated and subject to feedback regulation at different levels. In yeast, a central role is played by Sch9, a protein kinase that functions as a proximal eff...

α-Synuclein toxicity in yeast and human cells is caused by cell cycle re-entry ...

α-Synuclein (aSyn) toxicity is associated with cell cycle alterations, activation of DNA damage responses (DDR), and deregulation of autophagy. However, the relationships between these phenomena remain largely unknown. Here, we demonstrate that in a yeast model of aSyn toxicity and aging, aSyn expression induces Ras2-dependent growth signaling, cell cycle re-entry, DDR activation, autophagy, and autophagic degr...

New perspectives from South-Y-East, not all about death. A report of the 12th l...

Guidelines and recommendations on yeast cell death nomenclature

Elucidating the biology of yeast in its full complexity has major implications for science, medicine and industry. One of the most critical processes determining yeast life and physiology is cel-lular demise. However, the investigation of yeast cell death is a relatively young field, and a widely accepted set of concepts and terms is still missing. Here, we propose unified criteria for the defi-nition of accide...

pH homeostasis links the nutrient sensing PKA/TORC1/Sch9 ménage-à-trois to stre...

The plasma membrane H+-ATPase Pma1 and the vacuolar V-ATPase act in close harmony to tightly control pH homeostasis, which is essential for a vast number of physiological processes. As these main two regulators of pH are responsive to the nutritional status of the cell, it seems evident that pH homeostasis acts in conjunction with nutrient-induced signalling pathways. Indeed, both PKA and the TORC1-Sch9 axis in...

During the initiation of fermentation overexpression of hexokinase PII in yeast...

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:19:32Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:38:58Z : No. of bitstreams: 1 2-s2.0-0031891707.pdf: 380919 bytes, checksum: 1f8bc68e00355a68141ff9df472e503f (MD5); Made available in DSpace on 2014-05-27T11:19:33Z (GMT). No. of bitstreams: 0 Previous issue date: 1998-02-01; In the yeast Saccharomyces cerevisia...

Yeast stress, aging, and death

[Excerpt] Until about 15 years ago, programmed cell death (PCD), at that time mainly defined as apoptosis, was believed to be a feature occurring only in metazoans to ensure proper embryonic development, cell differentiation, and regulation of the immune response. However, the discovery that single-celled organisms, such as yeast, also undergo PCD challenged this idea. Meanwhile, several key regulators and cell...

SNCA (α-synuclein)-induced toxicity in yeast cells is dependent on sirtuin 2 (S...

SNCA (α-synuclein) misfolding and aggregation is strongly associated with both idiopathic and familial forms of Parkinson disease (PD). Evidence suggests that SNCA has an impact on cell clearance routes and protein quality control systems such as the ubiquitin-proteasome system (UPS) and autophagy. Recent advances in the key role of the autosomal recessive PARK2/PARKIN and PINK1 genes in mitophagy, highlighted ...

Synphilin-1 enhances α-synuclein aggregation in yeast and contributes to cellul...

Background: Parkinson’s disease is characterized by the presence of cytoplasmic inclusions, known as Lewy bodies, containing both aggregated α-synuclein and its interaction partner, synphilin-1. While synphilin-1 is known to accelerate inclusion formation by α-synuclein in mammalian cells, its effect on cytotoxicity remains elusive. Methodology/Principal Findings: We expressed wild-type synphilin-1 or its R621C...

Joined in death: highlights of the Sixth International Meeting on Yeast Apoptos...

While yeast apoptosis was still a controversial issue less than 10 years ago, the efforts of many groups have revealed cell death mechanisms that resemble, in many aspects, those described for mammalian apoptosis. Here, we provide an overview of new insights on yeast apoptosis and the link with lifespan of yeast cells, based on data presented at the 6th International Meeting of Yeast Apoptosis (IMYA). Together,...