3 documents found, page 1 of 1
Mechanism of action and the biological activities of Nigella sativa oil components
Silva, André Filipe C.; Haris, Parvez I.; Serralheiro, Maria Luisa; Pacheco, RitaNigella sativa seeds oil is consumed because of its health benefits. Although the oil has been studied for its properties and composition, these effects are often associated with its major compound thymoquinone, and less has been reported about the other components' contributions to the pharmacological properties and mechanism of action. The present study aimed to evaluate the oil compounds' antioxidant and ant...
Serum albumin modulates the bioactivity of rosmarinic acid
Brito, Elsa; Silva, André; Fale, Pedro; Pacheco, Rita; Serralheiro, António; Haris, Parvez I.; Ascensão, Lia; Serralheiro, Maria LuisaRosmarinic acid (RA) is a phenolic compound with biological activity. The objective of the present study was to investigate whether this compound kept its biological activity in the presence of proteins. For this purpose, bovine serum albumin (BSA) was used as a model protein, and the capacity of the RA to inhibit acetylcholinesterase (AChE) and affect antioxidant activity was evaluated in the absence and prese...
Substrate interaction with recombinant amidase from Pseudomonas aeruginosa duri...
Pacheco, Rita; Karmali, Amin; Serralheiro, Maria Luísa M.; Haris, Parvez I.The interaction of a variety of substrates with Pseudomonas aeruginosa native amidase (E.C. 3.5.1.4), overproduced in an Escherichia coli strain, was investigated using difference FTIR spectroscopy. The amides used as substrates showed an increase in hydrogen bonding upon association in multimers, which was not seen with esters. Evidence for an overall reduction or weakening of hydrogen bonding while amide and ...