22 documents found, page 1 of 3
Stem cells in end-to-side neurorrhaphy. Experimental study in rats
Paiva,Geruza Rezende; Viterbo,Fausto; Deffune,Elenice; Custódio,Maria Aparecida DominguesAbstract Purpose: To evaluate the influence of mesenchymal stem cells from adipose tissue in the end-to-side neurorrhaphy, focusing in the nerve regeneration and the muscle reinnervation in acute trauma. Methods: 140 animals were randomly divided in seven groups: control, denervated, end-to-side neurorrhaphy between distal stump of common peroneal nerve and tibial nerve (ESN), ESN wrapped in fascia, ESN wrapped...
Applicability of papain solutions in immunohematology
Santis,Laís Priscila De; Garcia,Patrícia Carvalho; Secco,Valéria Nogueira Dias Paes; Ferreira,Rosana Rossi; Deffune,EleniceABSTRACT Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences comp...
Explanation for the signs and symptoms of tooth eruption: mast cells
Franzolin,Solange de Oliveira Braga; Pardini,Maria Inês Moura Campos; Francischone,Leda A.; Deffune,Elenice; Consolaro,AlbertoAbstract This study contributes to the understanding of the mechanisms associated with signs and symptoms of tooth eruption, by investigating the presence of mast cells in pericoronal tissues during the intraosseous (Group 1) and submucosal (Group 2) phases of eruption. We compared findings for these two groups with each other and with those for the oral mucosa (Group 3). In each group, 14 specimens were analyz...
Isolation, cultivation and immunofluorescence characterization of lamellar kera...
Pfeifer,João P.H.; Santos,Vitor H.; Rosa,Gustavo; Souza,Jaqueline B.; Watanabe,Marcos Jun; Fonseca-Alves,Carlos E.; Deffune,Elenice; Alves,Ana L.G.ABSTRACT: The importance of the hoof to the horse health is clear, and the current knowledge regarding the cellular aspects of hoof keratinocytes is poor. Studies on equine keratinocyte culture are scarce. Developing keratinocyte cultures in vitro is a condition for studies on molecular biology, cell growth and differentiation. Some methods have already been established, such as those for skin keratinocyte cult...
ASSESSMENT OF KINETIC AND METABOLIC FEATURES OF TWO HYBRIDOMAS IN SUSPENSION CU...
Silva,Bruna Gabriela; Tamashiro,Wirla Maria da Silva Cunha; Ferreira,Rossana Rossi; Deffune,Elenice; Suazo,Cláudio Alberto TorresAbstract The aim of this study was to analyze kinetic and metabolic features of two hybridomas for monoclonal antibodies (MAbs) production for blood typing. Two lines of hybridomas, ED7 and ED9, were evaluated to produce anti-A and anti-AB, respectively. Experiments with ED7 and ED9 lines were conducted in a 500mL spinner flask and 2L bioreactor, using two different basal culture media, both with 10 % v/v addit...
Cyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-ind...
Lemos Neto,Sylvio Valença de; Vianna,Isabela Galvão; Castiglia,Yara Marcondes Machado; Golim,Marjorie de Assis; Souza,Aparecida Vitória Gonçalves deAbstract Purpose: To investigate the effects of cyclosporine A on renal ischemia-reperfusion injury during transient hyperglycemia in rats. Methods: In a model of ischemia-reperfusion-induced renal injury and transiently induced hyperglycemia by intraperitoneal injection of glucose, 2.5 g.kg-1, Wistar rats were anesthetized with either isoflurane or propofol and received intravenous cyclosporine A, 5 mg.kg-1, f...
Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold
Bertanha,Matheus; Sobreira,Marcone Lima; Bovolato,Ana Lívia de Carvalho; Rinaldi,Jaqueline de Carvalho; Reis,Patricia Pintor; Moroz,AndreiAbstract Purpose: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. Methods: Three groups were created for ultrastructural and residual DNA analysis: group 1 - control, consisting of samples of vena cava in natura; group 2 - SD, consisting of vein fragments submitted to 2% sodium deoxy...
Pilot study of homologous platelet gel in venous ulcers
Oliveira,Mariele Gobo de; Abbade,Luciana Patricia Fernandes; Miot,Hélio Amante; Ferreira,Rosana Rossi; Deffune,EleniceAbstract: Background: Venous ulcers represent 70% of the lower limb ulcers. They are difficult to heal, requiring a correct diagnostic and therapeutic approach. Many products have been developed to healing, such as homologous platelet gel obtained from the platelet concentrate exceeding from blood transfusion. Objective: To evaluate the safety and efficacy of homologous platelet gel in venous ulcers compared wi...
Platelet-rich plasma (PRP) applied during total knee arthroplasty
Guerreiro,João Paulo Fernandes; Danieli,Marcus Vinicius; Queiroz,Alexandre Oliveira; Deffune,Elenice; Ferreira,Rosana RossiOBJECTIVE: To evaluate the efficacy of platelet-rich plasma regarding healing, pain and hemostasis after total knee arthroplasty, by means of a blinded randomized controlled and blinded clinical study.METHODS: Forty patients who were going to undergo implantation of a total knee prosthesis were selected and randomized. In 20 of these patients, platelet-rich plasma was applied before the joint capsule was closed...
Light-emitting diode effects on combined decellularization of tracheae. A novel...
Evaristo,Thaiane Cristine; CruzAlves,Flávia Cilene Maciel da; Moroz,Andrei; Mion,Woner; Acorci-Valério,Michele Janegitz; Felisbino,Sérgio LuisPURPOSE: To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS:This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS:Generall...