ABSTRACT The objective of this study was to evaluate the effect of auxin pulse intervals on the induction of somatic embryos of Eucalyptus grandis x E. urophylla and to describe the embryogenic behavior of callus under the effect of auxinic stress. Cotyledons were inoculated in culture medium containing 207.07 µM picloram, a treatment considered as auxin pulse. Explants that were in the auxin pulse treatment we...
Abstract: Understanding the mechanisms involved in tree species maturation, related mainly to ontogenetic age effects, has contributed significantly to the vegetative propagation process of Brazilian native tree species, with consequent application of clonal forestry. A number of methodologies has been developed to rescue and vegetatively propagate these species for silvicultural and environmental restoration p...
ABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This ...
RESUMO O objetivo deste trabalho foi avaliar a produtividade de minicepas e o enraizamento de miniestacas de clones híbridos de Eucalyptus globulus em resposta a concentrações de nitrogênio em fertirrigação de minijardim clonal. Para isso, utilizaram-se cinco clones, sendo: dois clones de Eucalyptus urophylla x Eucalyptus globulus (C-04 e C-16); dois clones de Eucalyptus grandis x Eucalyptus globulus (C-26 e C-...
RESUMO Este trabalho objetivou avaliar o efeito de fontes de nitrogênio e respectivas doses sobre o crescimento e a qualidade de mudas de ipê-amarelo (Tabebuia serratifolia). As mudas foram produzidas em Latossolo Vermelho-Amarelo e as fontes de nitrogênio utilizadas foram nitrato de amônio, sulfato de amônio e nitrato de cálcio, aplicadas em cinco doses (0 mg, 75 mg, 150 mg, 225 mg e 300 mg dm–3) e parceladame...
ABSTRACT Flow cytometry and microsatellite markers were used to determine a genetic fidelity of micropropagated plants from the two Eucalyptus urophylla x E. globulus clones and a Eucalyptus grandis x E. globulus clone derived from adult material. Clones were repeatedly subcultured for 25 subcultures on MS medium supplemented with BA (2.22 µM) and ANA (0.05 µM) for in vitro shoot multiplication. The elongation ...
ABSTRACT The study aimed to evaluate the influence of storage length on Tectona grandis mini-cuttings survival and rooting. A factorial arrangement (4 x 7) was utilized, based on four clones (Carapá, Ipê, GU5 and TB7) and seven time intervals from mini-cuttings harvesting until final sowing (0, 1, 2, 4, 8, 12 and 16 hours). A randomized block design with three replicates and 16 mini-cuttings per experimental un...
ABSTRACT The objective of the present study was to test the effects of explant type, auxin concentrations, culture media, and auxin concentrations on the induction and proliferation of somatic embryos of Eucalyptus grandis x E. urophylla. Seeds and cotyledons were used as explants and inoculated in culture media containing 1.13, 2.26, 3.39 and 4.52 µM dicamba or 4.14, 10.35, 20.71 and 31.06 µM picloram. Embryog...
ABSTRACT This study aims to evaluate the effect of IBA concentrations and microcuttings successive collections in the micropropagation of Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones. Clumps containing six to eight buds of clones established in vitro were transferred to a 250 mL glass flask in JADS semisolid medium. Successive collections were performed every 20 days for Eucal...
Os híbridos de Eucalyptus globulus representam uma excelente alternativa para o setor de celulose e papel, em razão dos ganhos em qualidade da madeira para a fabricação de celulose. Entretanto, estes híbridos têm apresentado recalcitrância ao enraizamento adventício. Assim, a micropropagação é apontada como a técnica para o rejuvenescimento desses híbridos adultos, viabilizando a propagação clonal dos mesmos. O...