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Protocol for isolating nuclei from frozen fish heart tissue for single-cell gen...
Wei, Chaoguang; Jia, Ruonan; Canario, Adelino; Chen, Liangbiao; Xu, Qianghua; Hu, PengUnbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging. Here, we present a protocol for isolating high-quality nuclei from the heart tissue of Trematomus bernacchii, an Antarctic fish species with notoriously challenging tissue for nuclear extraction. We describe steps for pulverizing frozen hea...
Population genomics of an icefish reveals mechanisms of glacier-driven adaptive...
Lu, Ying; Li, Wenhao; Li, Yalin; Zhai, Wanying; Zhou, Xuming; Wu, Zhichao; Jiang, Shouwen; Liu, Taigang; Wang, Huamin; Hu, Ruiqin; Zhou, Yan; Zou, JunBackground Antarctica harbors the bulk of the species diversity of the dominant teleost fish suborder—Notothenioidei. However, the forces that shape their evolution are still under debate. Results We sequenced the genome of an icefish, Chionodraco hamatus, and used population genomics and demographic modelling of sequenced genomes of 52 C. hamatus individuals collected mainly from two East Antarctic regions to ...