11 documents found, page 1 of 2
WDR60-mediated dynein-2 loading into cilia powers retrograde IFT and transition...
De-Castro, ARG; Rodrigues, DRM; De-Castro, MJG; Vieira, N; Vieira, C; Carvalho, AX; Gassmann, R; Abreu, CMC; Dantas, TThe dynein-2 motor complex drives retrograde intraflagellar transport (IFT), playing a pivotal role in the assembly and functions of cilia. However, the mechanisms that regulate dynein-2 motility remain poorly understood. Here, we identify the Caenorhabditis elegans WDR60 homologue, WDR-60, and dissect the roles of this intermediate chain using genome editing and live imaging of endogenous dynein-2/IFT componen...
β-heavy-spectrin stabilizes the constricting contractile ring during cytokinesis
Silva, AM; Chan, FY; Norman, MJ; Sobral, AF; Zanin, E; Gassmann, R; Belmonte, JM; Carvalho, AXCytokinesis requires the constriction of an actomyosin-based contractile ring and involves multiple F-actin crosslinkers. We show that partial depletion of the C. elegans cytokinetic formin generates contractile rings with low F-actin levels that constrict but are structurally fragile, and we use this background to investigate the roles of the crosslinkers plastin/PLST-1 and β-heavy-spectrin/SMA-1 during ring c...
Equatorial Non-muscle Myosin II and Plastin Cooperate to Align and Compact F-ac...
Leite, J; Chan, FY; Osório, DS; Saramago, J; Sobral, AF; Silva, AM; Gassmann, R; Carvalho, AXCytokinesis is the last step of cell division that physically partitions the mother cell into two daughter cells. Cytokinesis requires the assembly and constriction of a contractile ring, a circumferential array of filamentous actin (F-actin), non-muscle myosin II motors (myosin), and actin-binding proteins that forms at the cell equator. Cytokinesis is accompanied by long-range cortical flows from regions of r...
PIG-1 MELK-dependent phosphorylation of nonmuscle myosin II promotes apoptosis ...
Wei, H; Lambie, EJ; Osório, DS; Carvalho, AX; Conradt, BThe mechanism(s) through which mammalian kinase MELK promotes tumorigenesis is not understood. We find that the C. elegans orthologue of MELK, PIG-1, promotes apoptosis by partitioning an anti-apoptotic factor. The C. elegans NSM neuroblast divides to produce a larger cell that differentiates into a neuron and a smaller cell that dies. We find that in this context, PIG-1 MELK is required for partitioning of CES...
Network contractility during cytokinesis-from molecular to global views
Leite, J; Osório, DS; Sobral, AF; Silva, AM; Carvalho, AXCytokinesis is the last stage of cell division, which partitions the mother cell into two daughter cells. It requires the assembly and constriction of a contractile ring that consists of a filamentous contractile network of actin and myosin. Network contractility depends on network architecture, level of connectivity and myosin motor activity, but how exactly is the contractile ring network organized or interco...
A transient helix in the disordered region of dynein light intermediate chain l...
Celestino, R; Henen, MA; Gama, JB; Carvalho, C; McCabe, M; Barbosa, DJ; Born, A; Nichols, PJ; Carvalho, AX; Gassmann, R; Vögeli, BAll animal cells use the motor cytoplasmic dynein 1 (dynein) to transport diverse cargo toward microtubule minus ends and to organize and position microtubule arrays such as the mitotic spindle. Cargo-specific adaptors engage with dynein to recruit and activate the motor, but the molecular mechanisms remain incompletely understood. Here, we use structural and dynamic nuclear magnetic resonance (NMR) analysis to...
NudE regulates dynein at kinetochores but is dispensable for other dynein funct...
Simões, PA; Celestino, R; Carvalho, AX; Gassmann, RIn mitosis, themolecular motor dynein is recruited to kinetochores by the Rod-Zw10-Zwilch complex (RZZ) and Spindly to control spindle assembly checkpoint (SAC) signaling and microtubule attachment. How the ubiquitous dynein co-factors Lis1 and NudE contribute to these functions remains poorly understood. Here, we show that the C. elegans NudE homolog NUD-2 is dispensable for dynein- and LIS-1-dependent mitotic...
TPXL-1 activates Aurora A to clear contractile ring components from the polar c...
Mangal, S; Sacher, J; Kim, T; Osório, DS; Motegi, F; Carvalho, AX; Oegema, K; Zanin, EDuring cytokinesis, a signal from the central spindle that forms between the separating anaphase chromosomes promotes the accumulation of contractile ring components at the cell equator, while a signal from the centrosomal microtubule asters inhibits accumulation of contractile ring components at the cell poles. However, the molecular identity of the inhibitory signal has remained unknown. To identify molecular...
Molecular mechanism of dynein recruitment to kinetochores by the Rod-Zw10-Zwilc...
Gama, JB; Pereira, C; Simões, PA; Celestino, R; Reis, RM; Barbosa, DJ; Pires, HR; Carvalho, C; Amorim, J; Carvalho, AX; Cheerambathur, DK; Gassmann, RThe molecular motor dynein concentrates at the kinetochore region of mitotic chromosomes in animals to accelerate spindle microtubule capture and to control spindle checkpoint signaling. In this study, we describe the molecular mechanism used by the Rod-Zw10-Zwilch complex and the adaptor Spindly to recruit dynein to kinetochores in Caenorhabditis elegans embryos and human cells. We show that Rod's N-terminal b...
Emerging Mechanisms and Roles for Asymmetric Cytokinesis
Thieleke-Matos, C; Osório, DS; Carvalho, AX; Morais-de-Sá, ECytokinesis completes cell division by physically separating the contents of the mother cell between the two daughter cells. This event requires the highly coordinated reorganization of the cytoskeleton within a precise window of time to ensure faithful genomic segregation. In addition, recent progress in the field highlighted the importance of cytokinesis in providing particularly important cues in the context...