6 documents found, page 1 of 1
Molecular characterization of Mycobacterium bovis infection in cattle and buffa...
CARNEIRO, P. A. M.; PASQUATTI, T. N.; TAKATANI, H.; ZUMÁRRAGA, M. J.; MARFIL, M. J.; BARNARD, C.; FITZGERALD, S. D.; ABRAMOVITCH, R. B.; ARAUJO, F. R.The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in cult...
Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry id...
BACANELLI, G.; OLARTE, L. C.; SILVA, M. R.; RODRIGUES, R. A.; CARNEIRO, P. A. M.; KANEENE, J. B.; PASQUATTI, T. N.; TAKATANI, H.; ZUMÁRRAGA, M. J.In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extr...
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with ...
BIGI, M.; VAZQUEZ, C. L.; CASTELÃO, A. B. C.; GARCÍA, E. A.; CATALDI, A. A.; JACKSON, M.; MAcNEIL, M.; SORIA, M.; ZUMÁRRAGA, M. J.; MATIAS, C.; GAGO, G.Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In...
Epidemiological Study of Mycobacterium bovis Infection in Buffalo and Cattle in...
CARNEIRO, P. A. M.; TAKATANI, H.; PASQUATTI, T. N.; SILVA, C. B. D. G.; NORBY, B.; WILKINS, M. J.; ZUMÁRRAGA, M. J.; ARAUJO, F. R.; KANEENE, J. B.Bovine Tuberculosis (BTB) is an endemic disease in about one hundred countries, affecting the economy causing a decrease in productivity, condemnation of meat, and damaging the credibility on international trade. Additionally, Mycobacterium bovis the major causative agent for BTB can also infect humans causing a variety of clinical presentations. The aim of this study was to determine BTB prevalence and the mai...
Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and ...
BIGI, M. M.; BLANCO, F. C.; ARAUJO, F. R.; THACKER, T. C.; ZUMÁRRAGA, M. J.; CATALDI, A. A.; SORIA, M. A.; BIGI, F.Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTCevolved. The genome of M. bovis is over>99.95% identical to that of M. tuberculosis but with seven deletions...
Patterns and Processes of Mycobacterium bovis Evolution Revealed by Phylogenomi...
PATANE, J. S. L.; MARTINS JUNIOR, J.; CASTELÃO, A. B.; NISHIBE, C.; MONTERA, L.; BIGI, F.; ZUMÁRRAGA, M. J.; CATALDI, A. A.; FONSECA JUNIOR, A.Mycobacterium bovis is an important animal pathogen worldwide that parasitizes wild and domesticated vertebrate livestock as well as humans. A comparison of the five M. bovis complete genomes from the United Kingdom, South Korea, Brazil, and the United States revealed four novel large-scale structural variations of at least 2,000 bp.Acomparative phylogenomic study including 2,483 core genes of 38 taxa from eigh...