25 documents found, page 1 of 3
INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL P...
Vilela,Marlúcia Souza Pádua; Andrade,Jéssica de Castro e; Santos,Raíssa Silveira; Stein,Vanessa Cristina; Alves,Patrick Callegari Magnani SantosABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oil palm plants in low concentrations of auxins and to observe the maintenance of these characteristics during in vi...
Molecular characterization of Bacillus thuringiensis strains to control Spodopt...
Machado,Déborah Heloísa Bittencourt; Livramento,Kalynka Gabriela do; Máximo,Wesley Pires Flausino; Negri,Bárbara França; Paiva,Luciano VilelaAbstract The main objective of this study was to characterize the toxicity and genetic divergence of 18 Bacillus thuringiensis strains in the biological control of Spodoptera eridania. Bacterial suspensions were added to the S. eridania diet. Half of the selected B. thuringiensis strains caused high mortality seven days after infection. The genetic divergence of B. thuringiensis strains was assessed based on En...
HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLA...
Vilela,Marlucia Souza Pádua; Andrade,Jessica de Castro e; Santos,Raissa Silveira; Stein,Vanessa Cristina; Paiva,Luciano VilelaABSTRACT Oil palm is economically important as a crop with high oil production. Indirect somatic embryogenesis in oil palm requires a long time for callus induction and plant formation, and it is important to study the embryogenic potential of calli and the mechanisms of somatic embryogenesis. The aim of this study was to test different growth regulators and spermine in induction of embryogenic calli in root ex...
In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor...
Máximo,Wesley Pires Flausino; Santos,Paulo Augusto Almeida; Martins,Guilherme Silva; Mendonça,Evânia Galvão; Paiva,Luciano VilelaAbstract This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) were the cytokinins used in the study. According to the r...
In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1
Pádua,Marlúcia Souza; Santos,Raíssa Silveira; Paiva,Luciano Vilela; Stein,Vanessa Cristina; Silva,Luciano CoutinhoABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germ...
USO DE BIORREATORES DE IMERSÃO CONTINUA, TEMPORÁRIA E MEIO DE CULTURA SEMISSÓLI...
Mendonça,Evânia Galvão; Stein,Vanessa Cristina; Carvalho,Humberto Henrique de; Santos,Breno Régis; Beijo,Luiz Alberto; Paiva,Luciano VilelaRESUMO A micropropagação em sistemas de biorreatores é considerada como uma forma de reduzir os custos de produção por meio do escalonamento de automatização do processo. O objetivo desse trabalho foi desenvolver um protocolo eficiente de produção de mudas de Eucalyptus camaldulensis em diferentes tipos de sistema, incluindo biorreator de imersão continua e temporária. Para isso, meristemas apicais (1 mm) e mer...
IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED ...
Abreu,José Renato de; Paiva,Luciano Vilela; Rodríguez,Miguel Angel Dita; Silva,Anderson Tadeu; Henriques,Ariadne Ribeiro; Chalfun-Junior,AntonioBrazil is the largest citrus producer in the world, being responsible for more than 20% of its production, which is, however still low due to phytosanitary issues such as citrus blight. Citrus blight is an anomaly whose causes still have not yet been determined, therefore there are no efficient control measures to minimize the production losses with the use of resistant varieties being considered the most appro...
Morphological characteristics and cell viability of coffee plants calli
Pádua,Marlúcia Souza; Paiva,Luciano Vilela; Silva,Luciano Coutinho da; Livramento,Kalynka Gabriella do; Alves,Eduardo; Castro,Ana Hortência FonsecaThe aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli sh...
Genetic transformation of Eucalyptus camaldulensis by agrobalistic method
Mendonça,Evânia Galvão; Stein,Vanessa Cristina; Balieiro,Flávia Pereira; Lima,Carolina Delfin Fernandes; Santos,Breno Régis; Paiva,Luciano VilelaEucalyptus stands in the setting of worldwide forestry due to its adaptability, rapid growth, production of high-quality and low cost of wood pulp fibers. The eucalyptus convetional breeding is impaired mainlly by the long life cycle making the genetic transformation systems an important tool for this purpose. However, this system requires in vitro eficient protocols for plant induction, regeneration and seleti...
Transcriptome analysis of resistant soybean roots infected by Meloidogyne javanica
Sá,Maria Eugênia Lisei de; Lopes,Marcus José Conceição; Campos,Magnólia de Araújo; Paiva,Luciano Vilela; Amorim,Regina Maria Santos deSoybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the transcriptome of soybean line PI 595099-Meloidogyne javanic...