4 documents found, page 1 of 1
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvem...
Cortez-Herrera,Elizabeth; Sperhacke,Rosa Dea; Becker,Daniela; Kritski,Afrânio; Zaha,Arnaldo; Rossetti,Maria Lucia RosaThe aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the ...
In search of essentiality: Mollicute-specific genes shared by twelve genomes
Souza,Rangel Celso; Almeida,Darcy Fontoura de; Zaha,Arnaldo; Morais,David Anderson de Lima; Vasconcelos,Ana Tereza Ribeiro deMollicutes are cell wall-less bacteria with a genome characterized by its small size. Chromosomal rearrangements help these organisms evade host immune surveillance and hence cause disease. Our goal was to determine genes shared by Mollicutes genomes using the bidirectional best hit methodology. The twelve studied Mollicutes share 210 genes, most of which (> 60%) fall into the following COG categories: translat...
Genes involved in cell division in mycoplasmas
Alarcón,Frank; Vasconcelos,Ana Tereza Ribeiro de; Yim,Lucia; Zaha,ArnaldoBacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes in...
Restriction-modification systems in Mycoplasma spp
Brocchi,Marcelo; Vasconcelos,Ana Tereza Ribeiro de; Zaha,ArnaldoRestriction and Modification (R-M) systems are present in all Mycoplasma species sequenced so far. The presence of these genes poses barriers to gene transfer and could protect the cell against phage infections. The number and types of R-M genes between different Mycoplasma species are variable, which is characteristic of a polymorphism. The majority of the CDSs code for Type III R-M systems and particularly fo...