Diatoms are photosynthetic microalgae with a siliceous cell wall and remarkable adaptability, making them a sustainable and renewable resource for high-value bioactive compounds (1,2). Their rapid growth, efficient carbon fixation, and minimal land and freshwater requirements (1,2) position them as attractive candidates for the development of eco-friendly products in the nutraceutical, pharmaceutical, and cosme...
Diatoms are microalgae with significant biotechnological potential due to their richness in bioactive compounds, including carotenoids and phenolics, contributing to their antioxidant properties. This study aims to compare the bioactive profile and antioxidant activity of three diatom species: Chaetoceros calcitrans, Nannofrustulum shiloi, and Cylindrotheca fusiformis cultivated in closed Flat Panel photobiorea...
Diatoms are a rich source of bioactive carotenoids, but their rigid silica frustules limit solvent penetration, reducing extraction efficiency and concealing the true carotenoid content. This study aimed to optimise the removal of silica from Chaetoceros calcitrans, Nannofrustulum shiloi, and Cylindrotheca fusiformis to enhance carotenoid extraction the efficiency. A combination of physical treatments (Ultratur...
Objective: This study evaluated the feasibility of using chocolate as a food matrix for delivering Akkermansia muciniphila, a next- generation probiotic known for improving gut barrier function and metabolic health. The research assessed the viability of A. muciniphila in three different chocolate formulations, characterised the chocolate’s biological characteristics, and evaluated the potential prebiotic and b...
Objective: To enhance the nutritional profile of a traditional Portuguese dish, esparregado, this project explored the incorporation of algae and microalgae as sustainable and health- promoting ingredients. Methods: A conventional esparregado recipe made with spinach was reformulated by incorporating algae-based ingredients. Three prototypes were developed: (1) with 10% (w/w) Ulva rigida; (2) with 10% (w/w) Chl...
Objective: This study aimed to develop and assess microalgae- enriched smoothies as stable, nutrient-rich, and sustainable functional beverages. The focus was on enhancing nutritional value and bioactive properties using two sustainably cultivated species: Chlorella vulgaris and Limnospira platensis (Spirulina). Methods: Two smoothie formulations were developed: SCv (with Chlorella vulgaris) and SSp (with Spiru...
Objective: The growing demand for plant-based diets and interest in ancestral crops have driven the development of innovative functional foods, such as plant-based ice creams. Akkermansia muciniphila, a next-generation probiotic, is notable for its therapeutic potential and its ability to modulate metabolic and inflammatory disorders, including obesity and metabolic syndrome. However, its incorporation into fro...
Introduction: Gut microbiota are the collection of bacteria, archaea and eukarya that colonise the gastrointestinal tract of animals and humans [1]. Prebiotics, probiotics and postbiotics have a positive e=ect on improving intestinal homeostasis by modulating both the microbiota profile and metabolic functions. This modulation may lead to beneficial changes in the production of specific organic acids and the co...
Noncommunicable diseases (NCDs), responsible for 41 million deaths each year, are often associated with unhealthy dietary habits. To combat this, nutrition and health organizations recommend a diet rich in fruits and vegetables (1). These foods are abundant in carotenoids, lipid-soluble phytochemicals (2) known for their health-enhancing properties, including antioxidant, anti-diabetic, and anti-mutagenic effec...
Legumes are essential to healthy agroecosystems, with a rich phytochemical content that impacts overall human and animal well-being and environmental sustainability. While these phytochemicals can have both positive and negative effects, legumes have traditionally been bred to produce genotypes with lower levels of certain plant phytochemicals, specifically those commonly termed as ‘antifeedants’ including phen...