9 documents found, page 1 of 1
Distribution and genetic diversity of tomato-infecting begomoviruses in Brazil
Ambrozevícius, L. P.; Calegario, R. F.; Fernandes, J. J.; Mello, R. N. de; Zerbini, F. M.; Ribeiro, S. G.; Ávila, A. C.; Bezerra, I. C.; Lima, M. F.Tomato-infecting begomoviruses have been reported throughout Brazil since the introduction of the B biotype of Bemisia tabaci. Here, we report a large scale survey on the distribution and genetic diversity of tomato-infecting begomoviruses. Tomato samples with typical begomovirus symptoms were collected in seven different states, comprising the major tomato growing areas of the country. Viruses were detected by...
New approach for the construction of infectious clones of a circular DNA plant ...
Xavier, C. A. D.; Zerbini, F. M.; Ferro, M. M. M.; Ramos-Sobrinho, R.; Lima, G. S. A.; Nagata, T.; Assunção, I. P.Viruses belonging to the genus Begomovirus (family Geminiviridae) have circular single-strand DNA genomes encapsidated into quasi-icosahedral particles, and are transmitted by whiteflies of the Bemisia tabaci complex. Biological and molecular properties of begomoviruses have been studied efficiently with infectious clones containing dimeric genomic components. However, current approaches employing enzymatic dig...
Three distinct begomoviruses associated with soybean in central Brazil
Fernandes, Fernanda R.; Cruz, A. R. R.; Faria, J. C.; Zerbini, F. M.; Aragão, Francisco J. L.We report the complete nucleotide sequences of geminiviruses of the genus Begomovirus infecting soybean (Glycine max) in central Brazil. Samples obtained from soybean plants collected at Santo Antonio de Goiás, Goiás State, showing typical symptoms of viral infection, were analyzed. Infection was confirmed by PCR-based amplification of a DNA-A fragment with universal begomovirus primers. Total DNA from infected...
Molecular variability of cowpea mild mottle virus infecting soybean in Brazil
Zanardo, L. G.; Silva, F. N.; Lima, A. T. M.; Milanesi, D. F.; Castilho-Urquiza, G. P.; Almeida, A. M. R.; Zerbini, F. M.; Carvalho, C. M.Molecular variability was assessed for 18 isolates of cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexiviridae) found infecting soybean in various Brazilian states (Bahia, Goias, Maranhao, ́ ) in 2001 and 2010. A Mato Grosso, Minas Gerais, Para variety of symptoms was expressed in soybean cv. CD206, ranging from mild (crinkle/blistering leaves, mosaic and vein clearing) to severe (bud blight, ...
A mosaic of beach bean (Canavalia rosea) caused by an isolate of cowpea aphid-b...
Madureira, P. M.; Kitajima, E. W.; Alcântara, B. K. de; Alfenas-Zerbini, P.; Rezende, J. A. M.; Zerbini, F. M.Beach bean (Canavalia rosea) plants showing mosaic symptoms were found at Massaguaçú beach, Caraguatatuba, Brazil. A potyvirus was found to be responsible for the symptoms, based on transmission assays and electron microscopy. A positive reaction in ELISA was obtained against cowpea aphid-borne mosaic (CABMV) antisera. Viral identity was confirmed by RT-PCR using specific primers to amplify part of the NIb and ...
Tomato yellow spot virus, a tomato-infecting begomovirus from Brazil with a clo...
Andrade, E. C.; Manhani, G. G.; Alfenas, P. F.; Calegario, R. F.; Fontes, E. P. B.; Zerbini, F. M.Geminiviruses are characterized by a circular, single-stranded DNA genome and twinned icosahedral particles. Begomoviruses (whitefly-transmitted geminiviruses) are a major constraint to crop production worldwide. In Brazil, tomato-infecting begomoviruses emerged as serious pathogens over the last 10 years, due to the introduction of a new biotype of the insect vector. Tomato yellow spot virus (ToYSV) is a newly...
Molecular characterisation and relative incidence of bean- and soybean-infectin...
Zerbini, F. M.; Rodrı́guez-Pardina, P. E.; Hanada, K.; Laguna, I. G.; Ducasse, D. A.Recent studies identified three begomoviruses infecting soybean and bean crops in northwestern (NW) Argentina, bean golden mosaic virus (BGMV), a virus with high capsid protein identity with Sida mottle virus (SiMoV) and a possible new viral species (isolate A). Analysis of complete DNA-A sequences confirmed that isolate A represents a new viral species for which the authors propose the name soybean blistering ...
Biological and molecular properties of Tomato rugose mosaic virus (ToRMV), a ne...
Fernandes, J. J.; Carvalho, M. G.; Andrade, E. C.; Brommonschenkel, S. H.; Fontes, E. P. B.; Zerbini, F. M.A viral complex causing golden mosaic and leaf distortion (rugosity) in tomato plants was obtained from viruliferous whiteflies, and named TGV-Ub1. This complex was sap-transmitted from tomato to Nicotiana benthamiana. PCR amplification using universal begomovirus primers yielded two distinct fragments for DNA-A, suggesting that the TGV-Ub1 complex comprised at least two distinct viruses. Clones corresponding t...
Molecular and biological characterization of Lettuce mosaic virus (LMV) isolate...
Krause-Sakate, R.; Le Gall, O.; Fakhfakh, H.; Peypelut, M.; Marrakchi, M.; Varveri, C.; Pavan, Marcelo Agenor [UNESP]; Souche, S.; Lot, H.Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:13:16Z No. of bitstreams: 0; Made available in DSpace on 2014-02-26T17:13:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2002-05-01; Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:21:09Z No. of bitstreams: 0; Made available in DSpace on 2014-05-20T13:21:09Z (GMT). No. of bitstreams: 0 ...