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Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from St...

Bes, Taniela Marli; Nagano, Debora Satie; Marchi, Ana Paula; Camilo, Gaspar; Perdigão-Neto, Lauro Vieira; Martins, Roberta Ruedas; Levin, Anna Sara

The methicillin resistant Staphylococcus aureus (MRSA) is recognized by its ability to acquire and transferring resistance genes through interspecies conjugative plasmids. However, transference of plasmids from Gram-positive cocci to Gram-negative bacilli is not well characterized. In this report, we describe the transfer of a conjugative plasmid carrying qacA from MRSA to Escherichia coli C600. We performed a ...

Date: 2021   |   Origin: Oasisbr

How new molecular tools can help bugbusters: a Burkholderia cepacia complex out...

Maciel, Amanda Luiz Pires; Schmitt, Cristiane; Baraldi, Marcia Maria; Silva, Caio de Lima; Oliveira, Luiz Felipe Valter de; Sampaio, Jorge Luiz Mello

An outbreak of bloodstream infection (BSI) caused by members of the Burkholderia cepacia complex (Bcc) took place from March 2012 until April 2014 involving thirteen patients. Aim. To describe an outbreak investigation of BSI Bcc and showing how genetic sequencing tools contributed to confirm the hypothesis of extrinsic contamination proposed by an observational study. Methods. The Infection Control Department ...

Date: 2020   |   Origin: Oasisbr

Zika virus infection among symptomatic patients from two healthcare centers in ...

Tozetto-Mendoza, Tania Regina; Avelino-Silva, Vivian Iida; Fonseca, Silvia; Claro, Ingra Morales; Paula, Anderson Vicente de; Levin, Anna Sara

Zika virus (ZIKV) clinical presentation and frequency/duration of shedding need further clarification. Symptomatic ZIKV-infected individuals identified in two hospitals in Sao Paulo State, Brazil, were investigated regarding clinical characteristics, shedding in body fluids, and serodynamics. Ninety-four of 235 symptomatic patients (Site A: 58%; Site B: 16%) had Real-Time PCR-confirmed ZIKV infection; fever, he...

Date: 2019   |   Origin: Oasisbr

Prevalence of methicillin-resistant Staphylococcus aureus colonization in indiv...

Bes, Taniela Marli; Martins, Roberta Ruedas; Perdigão, Lauro; Mongelos, Diego; Moreno, Luisa; Moreno, Andrea; Oliveira, Gerson Salvador de

Staphylococcus aureus (SA) is a commensal habitant of nasal cavities and skin. Colonization by community-acquired methicillin-resistant SA (CA-MRSA) is associated with infections in patients who have not been recently hospitalized. The aim of this study is to determine the prevalence of MRSA colonization in an outpatient population, currently unknown in Brazil. Three-hundred patients or caregivers from two teac...

Date: 2018   |   Origin: Oasisbr



INCIDENCE OF DIARRHEA BY Clostridium difficile IN HEMATOLOGIC PATIENTS AND HEMA...

Spadão, Fernanda; Gerhardt, Juliana; Guimarães, Thais; Dulley, Frederico; Almeida Junior, João Nóbrega de; Batista, Marjorie Vieira

Descrevemos a taxa de incidência de diarreia associada a Clostridium difficile (CDAD) em pacientes hematológicos e submetidos a transplante de células-tronco hematopoiéticas (TCTH) internados no HC-FMUSP no período de janeiro de 2007 a junho de 2011 usando dois denominadores 1.000 paciente e 1.000 dias de neutropenia e os fatores de risco associados à forma grave da doença e morte. O método de ELISA (Ridascreen...

Date: 2014   |   Origin: Oasisbr


Smqnr VARIANTS IN CLINICAL ISOLATES OF Stenotrophomonas maltophilia IN BRAZIL

Gracia-Paez, Jorge Isaac; Ferraz, Juliana Rosa; Franca E Silva, Ivan Avelino; Rossi, Flavia; Levin, Anna Sara; Costa, Silvia Figueiredo

SUMMARY Stenotrophomonas maltophilia contains a novel chromosomally-encoded qnr gene named Smqnr that contributes to low intrinsic resistance to quinolone. We described Smqnr in 13 clinical isolates of S. maltophilia from two Brazilian hospitals, over a 2-year period. The strains were identified by API 20 NE (bioMérieux, France). Susceptibility by microdilution method to trimetroprim/sulfamethoxazole, ciproflox...

Date: 2013   |   Origin: Oasisbr

Colonization pressure as a risk factor for colonization by multiresistant Acine...

DalBen, Mirian Freitas; Basso, Mariusa; Garcia, Cilmara Polido; Figueiredo Costa, Silvia; Maria Toscano, Cristiana; Robert Jarvis, William

OBJECTIVE: To determine factors associated with colonization by carbapenem-resistant Pseudomonas aeruginosa and multiresistant Acinetobacter spp. METHODS: Surveillance cultures were collected from patients admitted to the intensive care unit at admission, on the third day after admission and weekly until discharge. The outcome was colonization by these pathogens. Two interventions were implemented: education an...

Date: 2013   |   Origin: Oasisbr

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