5 documents found, page 1 of 1
PLK4 is a microtubule-associated protein that self-assembles promoting de novo ...
Montenegro Gouveia, Susana; Zitouni, Sihem; Kong, Dong; Duarte, Paulo; Ferreira Gomes, Beatriz; Sousa, Ana Laura; Tranfield, Erin M.; Hyman, AnthonyThe centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4, is critic...
Pericentrin-mediated SAS-6 recruitment promotes centriole assembly
Ito, Daisuke; Zitouni, Sihem; Jana, Swadhin Chandra; Duarte, Paulo; Surkont, Jaroslaw; Carvalho-Santos, Zita; Pereira-Leal, José BThe centrosome is composed of two centrioles surrounded by a microtubule-nucleating pericentriolar material (PCM). Although centrioles are known to regulate PCM assembly, it is less known whether and how the PCM contributes to centriole assembly. Here we investigate the interaction between centriole components and the PCM by taking advantage of fission yeast, which has a centriole-free, PCM-containing centrosom...
PLK4 is a microtubule-associated protein that self assembles promoting de novo ...
Gouveia, Susana Montenegro; Zitouni, Sihem; Kong, Dong; Duarte, Paulo; Gomes, Beatriz Ferreira; Sousa, Ana Laura; Tranfield, Erin M.; Hyman, AnthonyThe centrosome is an important microtubule-organizing centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4, is critic...
CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis
Zitouni, Sihem; Francia, Maria E.; Leal, Filipe; Montenegro Gouveia, Susana; Nabais, Catarina; Duarte, Paulo; Gilberto, Samuel; Brito, DanielaCentrioles are essential for the assembly of both centrosomes and cilia. Centriole biogenesis occurs once and only once per cell cycle and is temporally coordinated with cell-cycle progression, ensuring the formation of the right number of centrioles at the right time. The formation of new daughter centrioles is guided by a pre-existing, mother centriole. The proximity between mother and daughter centrioles was...
PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
Lopes, Carla A.M.; Jana, Swadhin Chandra; Cunha-Ferreira, Inês; Zitouni, Sihem; Bento, Inês; Duarte, Paulo; Gilberto, Samuel; Freixo, FranciscoCentrioles are essential for cilia and centrosome assembly. In centriole-containing cells, centrioles always form juxtaposed to pre-existing ones, motivating a century-old debate on centriole biogenesis control. Here, we show that trans-autoactivation of Polo-like kinase 4 (PLK4), the trigger of centriole biogenesis, is a critical event in the spatial control of that process. We demonstrate that centrioles prom...