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Rapid detection of Vancomycin-Resistant Enterococci (VRE) in rectal samples fro...

d'Azevedo, Pedro Alves; Santiago, Kelly Aline de Souza; Furtado, Guilherme Henrique Campos; Xavier, Diego Batista; Pignatari, Antonio Carlos Campos

ABSTRACT: The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR for detection and identification of vanA and ...

Date: 2011   |   Origin: Oasisbr

Molecular characterization of vancomycin-resistant Enterococci strains eight ye...

d'Azevedo, Pedro Alves; Furtado, Guilherme H.C.; Medeiros, Eduardo A.S.; Santiago, Kelly Aline; Silbert, Suzane; Pignatari, Antonio C.C.

E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures o...

Date: 2008   |   Origin: Oasisbr

Genetic diversity and antimicrobial resistance of enterococcal isolates from So...

d'Azevedo, Pedro Alves; Dias, Cícero Armídio Gomes; Teixeira, Lúcia Martins

In the present study, a total of 455 enterococcal isolates, recovered from patients living in the city of Porto Alegre, State of Rio Grande do Sul, Brazil, during the period from July 1996 to June 1997, were identified to the species level by conventional biochemical and microbiological tests, and assayed for their susceptibilities to antimicrobial agents. The genetic diversity of antimicrobial resistant strain...

Date: 2006   |   Origin: Oasisbr

Candidemia in a Brazilian tertiary care hospital: species distribution and anti...

Antunes, Ana Graciela Ventura; Pasqualotto, Alessandro Comarú; Diaz, María Cristina; d'Azevedo, Pedro Alves; Severo, Luiz Carlos

Recent studies have shown differences in the epidemiology of invasive infections caused by Candida species worldwide. In the period comprising August 2002 to August 2003, we performed a study in Santa Casa Complexo Hospitalar, Brazil, to determine Candida species distribution associated with candidemia and their antifungal susceptibility profiles to amphotericin B, fluconazole and itraconazole. Antifungal susce...

Date: 2004   |   Origin: Oasisbr

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