3 documents found, page 1 of 1
Copper-64 Chloride Exhibits Therapeutic Potential in Three-Dimensional Cellular...
Pinto, Catarina I. G.; Bucar, Sara; Alves, Vítor H. P.; Fonseca, Alexandra; Abrunhosa, Antero J.; da Silva, Cláudia L.; Guerreiro, Joana F.Prostate cancer (PCa) is the second most common cancer type in men, and in advanced metastatic stages is considerable incurable. This justifies the need for efficient early diagnostic methods and novel therapies, particularly radiopharmaceuticals with the potential for simultaneous diagnosis and therapy (theranostics). We have previously demonstrated, using monolayer-cultured cells, that copper-64 chloride, a p...
Impact of the human mesenchymal stem cells donor on conditional medium composition
Pereira, Maria João Canha; Ramalhete, Luís; Aleixo, Sandra; da Silva, Cláudia L.; Cabral, Joaquim M.S.; Calado, Cecília; Fernandes, AnaExosomes produced by Mesenchymal Stem Cells (MSCs) can represent a very appealing strategy for cell-free therapies. However, to achieve this reality it is necessary to further understand the process associated to the MSC culture when conditioned to produce exosomes. In the present work, it was evaluated how different MSC obtained from different donors may affect the conditioned media composition and how this ca...
Developing a co-culture system for effective megakaryo/thrombopoiesis from umbi...
Hatami, Javad; Andrade, Pedro Z.; Alves de Matos, António Pedro; Djokovic, Dusan; Lilaia, Carla; Ferreira, Frederico Castelo; Cabral, Joaquim M. S.Background aims. Platelet transfusion can be a life-saving procedure in different medical settings. Thus, there is an increasing demand for platelets, of which shelf-life is only 5 days. The efficient ex vivo biomanufacturing of platelets would allow overcoming the shortages of donated platelets. Methods. We exploited a two-stage culture protocol aiming to study the effect of different parameters on the megakar...