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Heterologous expression of predicted promoter site for paraquat-inducible genes...

Gabriel, Jane Eyre; de Souza, Emanuel Maltempi; de Carvalho, Francisco Allan Leandro; Madeira, Humberto Maciel França

The aim of this study was to evaluate functionally the effect of plumbagin on the heterologous expression of a predicted promoter region of open reading frames of paraquat-inducible (pqi) genes revealed during genome annotation analyses of the bacterium Chromobacterium violaceum. First, the promoter of interest was amplified using specific primers and cloned into a conjugative vector carrying the Escherichia co...

Date: 2017   |   Origin: Oasisbr
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