4 documents found, page 1 of 1
Impact of grape pectic polysaccharides on anthocyanins thermostability
Fernandes, Ana; Brandão, Elsa; Raposo, Filomena; Maricato, Élia; Oliveira, Joana; Mateus, Nuno; Coimbra, Manuel A; de Freitas, VictorThe impact of grape pectic polysaccharides on malvidin-3-O-β-d-glucoside thermostability was evaluated in model solutions. Pectic polysaccharides richer in homogalacturonan domains, with less neutral sidechains (chelator fraction) showed higher binding with malvidin-3-O-β-d-glucoside, by 1H NMR (Ka=505 M-1). Binding affinity with water soluble extract was estimated to be 10-fold lower, possibly due to the prese...
Sulfate-based lipids: analysis of healthy human fluids and cell extracts
Dias, Irundika H. K.; Ferreira, Rita; Gruber, Florian; Vitorino, Rui; Rivas-Urbina, Andrea; Sanchez-Quesada, José Luis; Vieira Silva, JoanaSulfate-based lipids (SL) have been proposed as players in inflammation, immunity and infection. In spite of the many biochemical processes linked to SL, analysis on this class of lipids has only focused on specific SL sub-classes in individual fluids or cells leaving a range of additional SL in other biological samples unaccounted for. This study describes the mass spectrometry screening of SL in lipid extract...
Quercetin protects Saccharomyces cerevisiae against oxidative stress by inducin...
Vilaça, Rita; Mendes, Vanda; Mendes, Marta Vaz; Carreto, Laura; Amorim, Maria Amélia; de Freitas, Victor; Moradas-Ferreira, Pedro; Mateus, NunoBackground: Quercetin is a naturally occurring flavonol with antioxidant, anticancer and anti-ageing properties. In this study we aimed to identify genes differentially expressed in yeast cells treated with quercetin and its role in oxidative stress protection. Methods: A microarray analysis was performed to characterize changes in the transcriptome and the expression of selected genes was validated by RT-qPCR....
Anti-tumoral activity of imidazoquines, a new class of antimalarials derived fr...
Fernandes, Iva; Vale, Nuno; de Freitas, Victor; Moreira, Rui; Mateus, Nuno; Gomes, PaulaThe growth inhibitory activity of imidazoquines, antimalarial imidazolidin-4-ones derived from primaquine, on human cancer cell lines HT-29, Caco-2, and MCF-7 has been evaluated. Primaquine, N-dipeptidyl-primaquine derivatives, and other quinolines have been included in the study for comparison purposes. Primaquine and some of its derivatives were significantly active against the MCF-7 human breast cancer cell ...