10 documents found, page 1 of 1
Structural Aspects of the Distinct Biochemical Properties of Glutaredoxin 1 and...
Discola, Karen Fulan; de Oliveira, Marcos Antonio [UNESP]; Rosa Cussiol, José Renato; Monteiro, Gisele; Bárcena, José Antonio; Porras, PabloMade available in DSpace on 2022-04-28T20:48:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-01-23; Glutaredoxins (Grxs) are small (9-12 kDa) heat-stable proteins that are ubiquitously distributed. In Saccharomyces cerevisiae, seven Grx enzymes have been identified. Two of them (yGrx1 and yGrx2) are dithiolic, possessing a conserved Cys-Pro-Tyr-Cys motif. Here, we show that yGrx2 has a specific activi...
Expression of glycosylated proteins in bacterial system and purification by aff...
Breyer, Carlos Alexandre [UNESP]; de Oliveira, Marcos Antonio [UNESP]; Pessoa, AdalbertoMade available in DSpace on 2022-04-29T08:14:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01; The bacterial expression of glycoproteins has experienced significant progress in recent years, particularly in regard to the production of conjugate vaccines against pathogens. In this case, a protein carrier conjugated with glycosides is used to produce intense stimulation of the immune system. Glycoc...
Effects of serine or threonine in the active site of typical 2-cys prx on hyper...
Tairum, Carlos A. [UNESP]; Santos, Melina Cardoso [UNESP]; Breyer, Carlos Alexandre [UNESP]; de Oliveira, Ana Laura Pires [UNESP]Made available in DSpace on 2022-05-01T05:29:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Typical 2-Cys peroxiredoxins (2-Cys Prx) are ubiquitous Cys-based peroxidases, which are stable as decamers in the reduced state, and may dissociate into dimers upon disulfide bond formation. A peroxidatic Cys (CP) takes part of a catalytic ...
Structural and functional diversity of asparaginases: Overview and recommendati...
da Silva, Leonardo Schultz [UNESP]; Doonan, Liam B.; Pessoa, Adalberto; de Oliveira, Marcos Antonio [UNESP]; Long, Paul F.Made available in DSpace on 2021-06-25T10:26:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01; Asparaginases (ASNases) are a large and structurally diverse group of enzymes ubiquitous amongst archaea, bacteria and eukaryotes, that catalyze hydrolysis of asparagine to aspartate and ammonia. Bacterial ASNases are important biopharmaceuticals for the treatment of acute lymphoblastic leukemia, althou...
Functional and structural evaluation of the antileukaemic enzyme l-asparaginase...
de Moura, Werner Alfinito Feio [UNESP]; Schultz, Leonardo [UNESP]; Breyer, Carlos Alexandre [UNESP]; de Oliveira, Ana Laura Pires [UNESP]Made available in DSpace on 2020-12-12T02:45:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01; Acute lymphoblastic leukaemia (ALL) affects lymphoblastic cells and is the most common neoplasm during childhood. Among the pharmaceuticals used in the treatment protocols for ALL, Asparaginase (ASNase) from Escherichia coli (EcAII) is an essential biodrug. Meanwhile, the use of EcAII in neoplastic trea...
Reduction of sulfenic acids by ascorbate in proteins, connecting thiol-dependen...
Anschau, Valesca; Ferrer-Sueta, Gerardo; Aleixo-Silva, Rogerio Luis; Bannitz Fernandes, Renata; Tairum, Carlos A.; Tonoli, Celisa Caldana CostaMade available in DSpace on 2020-12-12T02:15:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-20; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Universidad de la República Uruguay; Sulfenic acids are the primary product of thiol oxidation by hydrogen peroxide and other oxidants. Several aspects of sulfenic acid for...
Bioaffinity fishing procedure using secretory phospholipase A2 for screening fo...
dos Santos, Adeilso Bispo [UNESP]; Tamayose, Cinthia I.; Ferreira, Marcelo J.P.; Belchor, Mariana N. [UNESP]; Costa, Caroline R.C. [UNESP]Made available in DSpace on 2020-12-12T01:53:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identificat...
Glutaredoxin-like protein (GLP)—a novel bacteria sulfurtransferase that protect...
de Paula, Carla Peres [UNESP]; dos Santos, Melina Cardoso [UNESP]; Tairum, Carlos A. [UNESP]; Breyer, Carlos Alexandre [UNESP]; Toledo-Silva, GuilhermeMade available in DSpace on 2020-12-12T02:04:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); The pathogen Xylella fastidiosa belongs to the Xanthomonadaceae family, a large group of Gram-negative bacteria that cause diseases in many economically important crops. A predicted gene, annotated as glutaredoxin-like protein (glp), was fou...
Evaluation of the Inhibitory Potential of Casuarictin, an Ellagitannin Isolated...
Rodrigues, Caroline Fabri Bittencourt [UNESP]; Ferreira, Marcelo José Pena; Belchor, Mariana Novo [UNESP]; Costa, Caroline R.C. [UNESP]Made available in DSpace on 2019-10-06T16:38:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01; National Council for Scientific Research; Universidade de São Paulo; Ellagitannins constitute the largest group of hydrolyzable tannins of plants, and, from this group, casuarictin (Casu) was identified in some plant species. However, to our knowledge, no investigation of secretory phospholipase A2 (sPL...
Diversity and Distribution of Heavy Metal-Resistant Bacteria in Polluted Sedime...
Zampieri, Bruna Del Busso [UNESP]; Pinto, Aline Bartelochi [UNESP]; Schultz, Leonardo [UNESP]; de Oliveira, Marcos Antonio [UNESP]Made available in DSpace on 2018-12-11T17:04:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01; Heavy metals influence the population size, diversity, and metabolic activity of bacteria. In turn, bacteria can develop heavy metal resistance mechanisms, and this can be used in bioremediation of contaminated areas. The purpose of the present study was to understand how heavy metals concentration infl...