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Influence of Feeding Weaned Piglets with Laminaria digitata on the Quality and ...

Ribeiro, David; Alfaia, Cristina; Pestana, José; Carvalho, Daniela; Costa, Mónica; Martins, Cátia; Lemos, José; Mourato, Miguel; Gueifão, Sandra

Laminaria digitata is a novel feedstuff that can be used in pig diets to replace conventional feedstuffs. However, its resilient cell wall can prevent the monogastric digestive system from accessing intracellular nutrients. Carbohydrate-active enzyme (CAZyme) supplementation is a putative solution for this problem, degrading the cell wall during digestion. The objective of this work was to evaluate the effect o...


Infuence of Chlorella vulgaris on growth, digestibility and gut morphology and ...

Martins, Cátia Falcão; Trevisi, Paolo; Coelho, Diogo F.; Correa, Federico; Ribeiro, David M.; Alfaia, Cristina; Alfaia, Cristina; Alfaia, Cristina

Research Areas: Science & Technology; The purpose of this study was to evaluate the impact of Chlorella vulgaris (5% in the diet), supplemented or not with two exogenous carbohydrase mixtures on piglets’ performance, nutrient digestibility and gut morphology, fermentation products and microbiota. Forty-four male piglets weaned at 28 days of age, with 11.2 ± 0.46 kg of live weight, were used and assigned to 1 of...


Effect of Dietary Laminaria digitata with Carbohydrases on Broiler Production P...

Costa, M.M.; Pestana, José; Osório, Diogo; Alfaia, Cristina; Martins, Cátia Falcão; Mourato, M.; Rego, Andreia; Lemos, J. P. C.; Fontes, Carlos M.G.A.

Research Areas: Agriculture; Veterinary Sciences; Zoology; ABSTRACT - We hypothesized that dietary inclusion of 15% Laminaria digitata, supplemented or not with carbohydrases, could improve the nutritional value of poultry meat without impairing animal growth performance. A total of 120 22-day old broilers were fed the following dietary treatments (n = 10) for 14 days: cereal-based diet (control); control diet ...


Impact of Chlorella vulgaris as feed ingredient and carbohydrases on the health...

Coelho, Diogo; Alfaia, Cristina; Lopes, Paula Alexandra; Pestana, José M.; Costa, Monica M.; Pinto, Rui M.; Almeida, Joao M.; Moreira, Olga

Research Areas: Veterinary Sciences; The implication of high dietary level of Chlorella vulgaris, individually and supplemented with two carbohydrase mixtures, on pigs' health and liver metabolism was assessed in this study. Forty crossbred (Large White × Landrace sows crossed with Pietrain boars) entire male pigs were randomly allocated to the following feeding treatments (n = 10): cereal-soybean meal basal di...


Combined efects of dietary Laminaria digitata with alginate lyase on plasma met...

Costa, Mónica; Lopes, Paula Alexandra; Assunção, JMP; Alfaia, Cristina; Coelho, Diogo; Mourato, Miguel; Pinto, Rui; Lordelo, Maria; Prates, JAM

Research Areas: Veterinary Sciences; ABSTRACT - Background: The Laminaria digitata is an abundant macroalga and a sustainable feedstock for poultry nutrition. L. digitata is a good source of essential amino acids, carbohydrates and vitamins, including A, D, E, and K, as well as triacylglycerols and minerals, in particular iron and calcium. However, the few studies available in the literature with broilers docum...


Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan ly...

Costa, Monica; Pio, Luís Bernardo; Bule, Pedro; Duarte, Marlene; Alfaia, Cristina; Coelho, Diogo; Bras, Joana; Fontes, Carlos M.G.A.; Prates, José A.M

Research Areas: Agriculture; Veterinary Sciences; ABSTRACT - Green macroalgae, e.g., Ulva lactuca, are valuable bioactive sources of nutrients; but algae recalcitrant cell walls, composed of a complex cross-linked matrix of polysaccharides, can compromise their utilization as feedstuffs for monogastric animals. This study aimed to evaluate the ability of pre-selected Carbohy- drate-Active enZymes (CAZymes) and ...


Total lipids, fatty acid composition, total cholesterol and lipid-soluble antio...

da Silva, Jamile Andrea Rodrigues; Rodrigues, Laurena Silva; Lourenco-Junior, Jose de Brito; Alfaia, Cristina; Costa, Monica Mendes

Research Areas: Agriculture ; Veterinary Sciences ; Zoology; The aim of this study was to analyze the influence of distinct production systems and seasonal variation in the Brazilian Eastern Amazon on the meat lipid composition of water buffaloes. Water buffaloes were reared in commercial farms in the Eastern Amazon either in extensive systems (Marajó Island, Nova Timboteua and Santarém locations), during rainy...


Combined effects of dietary Laminaria digitata with alginate lyase on plasma me...

Costa, Mónica Mendes; Lopes, Paula Alexandra; Assunção, José Miguel Pestana; Alfaia, Cristina; Coelho, Diogo; Mourato, Miguel; Pinto, Rui Manuel

Background: The Laminaria digitata is an abundant macroalga and a sustainable feedstock for poultry nutrition. L. digitata is a good source of essential amino acids, carbohydrates and vitamins, including A, D, E, and K, as well as triacylglycerols and minerals, in particular iron and calcium. However, the few studies available in the literature with broilers document the application of this macroalga as a dieta...


Influence of dietary Chlorella vulgaris and carbohydrate-active enzymes on grow...

Alfaia, Cristina; Pestana, J.M.; Rodrigues, M.; Coelho, D.; Aires, M.J.; Ribeiro, D.M.; Major, V. T.; Martins, C. F.; Lemos, J.P.C.; Fontes, M.G.A.

Research Areas: Agriculture, Dairy & Animal Science; Herein, we investigated the effect of Chlorella vulgaris as ingredient (10% of incorporation) in broiler diets, supplemented or not with 2 formulations of Carbohydrate-Active enZymes (CAZymes; Rovabio Excel AP and a mixture of recombinant CAZymes, composed by an exo-beta-glucosaminidase, an alginate lyase, a peptidoglycan N-acetylmuramic acid deacetylase and ...


Effects of Chlorella vulgaris as a feed ingredient on the quality and nutrition...

Martins, Cátia Falcão; Pestana, José M.; Alfaia, Cristina; Costa, Mónica; Ribeiro, D. M.; Coelho, Diogo; Lopes, Paula Alexandra; Almeida, André

Research Areas: Food Science & Technology; Chlorella vulgaris (CH) is usually considered a feed supplement in pig nutrition, and its use as an ingredient is poorly studied. Among many interesting characteristics, this microalga has high protein levels and can be a putative alternative for soybean meal. Our aim was to study the effect of a 5% CH incorporation in the diet, individually or combined with two carboh...


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