5 documents found, page 1 of 1
Molecular typing of Mycobacterium bovis isolated in the south of Brazil
Ramos,Daniela Fernandes; Silva,Ana Bárbara Scholante; Fagundes,Michel Quevedo; von Groll,Andrea; Silva,Pedro Eduardo Almeida daBovine tuberculosis is a major infectious disease of the cattle. In this study, 85 M. bovis isolates from 162 lymph nodes, obtained from a herd of cattle on a farm in southern Brazil, were evaluated using spoligotyping and VNTR. The strains were grouped into five clusters and five orphans, showing a heterogenic genetic profile, what could represent diverse geographic origins of the introduced cows and/or the fr...
Mycobacterium tuberculosis belonging to family LAM and sublineage RD Rio: commo...
Soares,Renata Oliveira; Macedo,Maíra Bidart de; von Groll,Andrea; Silva,Pedro Eduardo Almeida daA sublineage of Mycobacterium tuberculosis called RD Rio was described in 2007. Although only recently described, this strain may have been present previously in the population, and its identification in clinical isolates will elucidate bacterial transmission dynamics and host-pathogen interactions. This study evaluated the clonal diversity of the RD Rio sublineage in clinical isolates from Rio Grande-RS obtain...
Improving tuberculosis control through the partnership between university and t...
Valença,Mariana Soares; Rocha,Jeane Zanini da; Ramis,Ivy Bastos; Carrion,Lillian Lucas; Madruga,Catiúcia; Macedo,Maíra Bidart de; Scaini,Carlos JamesINTRODUCTION: Tuberculosis (TB) control is linked to the availability of qualified methods for microbiological diagnostics; however, microscopy with limited sensitivity is the only method available in many locations. The objective of this study was to evaluate the introduction of culture, drug susceptibility testing (DST), and genotyping in the routine of a Municipal Program of Tuberculosis Control. METHODS: Di...
Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazuri...
von Groll,Andrea; Martin,Anandi; Portaels,Françoise; Silva,Pedro Eduardo Almeida da; Palomino,Juan CarlosWe standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.
Comparative evaluation of the Nitrate Reductase Assay and the Resazurin Microti...
Sanchotene,Karine O.; von Groll,Andrea; Ramos,Daniela; Scholante,Ana B.; Honscha,Gunther; Valença,Mariana; Scaini,Carlos J.; Silva,Pedro E.A. daTuberculosis remains as a serious infection disease of worldwide distribution, with high morbidity and mortality, mainly in low socio-economic condition countries. The state of emergency of tuberculosis caused by the resistant and multidrug-resistant (MDR) strains, became the main threat to the tuberculosis treatment and control programs. A fast detection method for the resistant strains will allow the implemen...