Author(s): Meirinho, Sofia G. ; Dias, L.G. ; Peres, António M. ; Rodrigues, Lígia R.
Date: 2014
Persistent ID: http://hdl.handle.net/10198/15823
Origin: Biblioteca Digital da UPB
Author(s): Meirinho, Sofia G. ; Dias, L.G. ; Peres, António M. ; Rodrigues, Lígia R.
Date: 2014
Persistent ID: http://hdl.handle.net/10198/15823
Origin: Biblioteca Digital da UPB
Electrochemical RNA aptasensor To obtain bio-sensor with high affinity for rhOPN aiming to be used for cancer prognosis Materials and methods RNA aptamer details Isolated by SELEX process [1] Sequence of the biotinylated RNA aptamer 5’-Biotin- CGGCCACAGAAUGAAAAACCUCAUCGAUGUUGCAUAGUUG-3’ Removal the interference of Rnase Working RNA aptamer solution 4 nM in PBS. RNA aptamer immobilization on a gold surfasse via streptavidin-biotin interaction